State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Multiscale Research Institute of Complex Systems, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, China.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China.
Nat Commun. 2021 May 25;12(1):3089. doi: 10.1038/s41467-021-23407-2.
Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding "on" or "off" SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity.
具有可逆荧光的光致变色探针彻底改变了单分子光谱学和超分辨率显微镜领域，但缺乏足够的化学特异性。相比之下，具有受激拉曼散射（SRS）显微镜的喇曼探针为超多重成像提供了极好的化学分辨率，但相对惰性。在这里，我们通过工程炔基标记二芳乙烯来报告振动光致变色，以实现光致可切换的 SRS 成像。当用紫外线（UV）或可见光照射时，共轭二芳乙烯的光致异构化会使炔基基团的窄拉曼峰可逆位移，从而在光活性拉曼频率处获得“开”或“关”的 SRS 图像。我们在薄膜上演示了可重写的图案和加密、用标记炔基-二芳乙烯对细胞进行涂写/擦除，以及活细胞中线粒体扩散的脉冲追踪实验。该设计原理为具有振动特异性的超分辨率显微镜、光学存储器和开关提供了潜力。
