Medical Laboratory Technology Department, College of Medical Technology, the Islamic University, Najaf, Iraq.
College of Medicine, University of Al-Ameed, Karbala, Iraq.
Arch Razi Inst. 2022 Feb 28;77(1):461-466. doi: 10.22092/ARI.2021.356811.1918. eCollection 2022 Feb.
Adult males are considered the main causes of infertility defects in the world; therefore, scientists are searching for factors that play a role in male fertility, such as kisspeptin, which acts as one of the master controllers and regulators of gonadotropin secretion from the hypothalamus in mammalian species. Furthermore, it has been shown that the kisspeptin receptor is mainly localized in the interstitial and germ cells of the testicles and has a role in the regulation of gonadal development and function. This study aimed to investigate the effect of kisspeptin on testicular degeneration syndrome induced by cadmium chloride in male Wistar rats and identify the best concentration of kisspeptin to cure this syndrome. A total of 40 male rats were divided into four equal groups of negative control (n=10) that was injected subcutaneously (SC) with normal saline solution twice a week for 42 days; positive control (n=10) that was intraperitoneally injected with cadmium chloride (1 mg/kg B.W., once a week for two weeks); T that was intraperitoneally injected with cadmium chloride (1 mg/kg B.W., once a week for two weeks), and after 14 days, 20 nmol/rat of kisspeptins were injected SC twice a week for 42 days in all animals; and T that was intraperitoneally injected with cadmium chloride (1 mg/kg B.W. once a week for two weeks), and after 14 days, 40 nmol/rat of kisspeptins were injected SC twice a week for 42 days in all animals. All animals were then euthanized after 42 days, and their testes were dissected for a histological study. The results of the present study confirmed that cadmium chloride affected the male testes and sperm parameters, while the administration of kisspeptin at doses of 20 and 40 nmol/rat (twice a week) by SC injection showed a significant effect in restoring the reproduction feature and histology of the rats' testicular tissue.
成年男性被认为是世界范围内导致不育缺陷的主要原因；因此，科学家们一直在寻找影响男性生育能力的因素，如 kisspeptin，它是哺乳动物下丘脑促性腺激素分泌的主要控制器和调节剂之一。此外，已经表明 kisspeptin 受体主要定位于睾丸的间质细胞和生殖细胞中，在调节性腺发育和功能方面发挥作用。本研究旨在探讨 kisspeptin 对氯化镉诱导的雄性 Wistar 大鼠睾丸退行性综合征的影响，并确定 kisspeptin 的最佳浓度来治疗这种综合征。将 40 只雄性大鼠随机分为 4 组，每组 10 只：阴性对照组（n=10），每周两次皮下注射生理盐水 42 天；阳性对照组（n=10），每周一次腹腔注射氯化镉（1mg/kg B.W.，连续两周）；T 组，每周一次腹腔注射氯化镉（1mg/kg B.W.，连续两周），14 天后，所有动物每周两次皮下注射 20nmol/rat kisspeptin 42 天；T 组，每周一次腹腔注射氯化镉（1mg/kg B.W.，连续两周），14 天后，所有动物每周两次皮下注射 40nmol/rat kisspeptin 42 天。所有动物在 42 天后处死，解剖睾丸进行组织学研究。本研究结果证实，氯化镉影响雄性睾丸和精子参数，而通过皮下注射给予 kisspeptin 剂量为 20 和 40nmol/rat（每周两次），对恢复大鼠睾丸组织的生殖特征和组织学有显著效果。
