Vera-Baquero Fernando L, Morante-Zarcero Sonia, Sierra Isabel
Departamento de Tecnología Química y Ambiental, E.S.C.E.T., Universidad Rey Juan Carlos, C/Tulipán s/n, Móstoles, 28933 Madrid, Spain.
Foods. 2022 Jul 23;11(15):2196. doi: 10.3390/foods11152196.
In this work, the thermal degradation of tropane and opium alkaloids was studied in samples of breadsticks prepared with corn flour, contaminated with seeds of , and containing seeds of L. A total of seven different samples were prepared and eight alkaloids were studied, three tropane (atropine, scopolamine, and anisodamine) and five opium (morphine, codeine, thebaine, papaverine, and noscapine) alkaloids. For this purpose, a fast, easy and efficient method based on solid-liquid extraction (SLE) prior to the analysis by high-performance liquid chromatography with a diode array detector (HPLC-DAD) was developed and validated. Thermal degradation studies showed a decrease in the TAs and OAs content under baking (180 °C for 20 min) that was between 7-65% for atropine, depending on the preparation conditions used, between 35-49% for scopolamine and anisodamine, up to 100% for morphine and codeine and between 14-58% for thebaine, papaverine, and noscapine. Results also evidenced that degradation of morphine and codeine was higher when the seeds were added as topping to the breadsticks.
在这项工作中，研究了用玉米粉制备的面包棒样品中托烷生物碱和鸦片生物碱的热降解情况，这些样品被 种子污染，并含有 种子。总共制备了七个不同的样品，并研究了八种生物碱，三种托烷生物碱（阿托品、东莨菪碱和山莨菪碱）和五种鸦片生物碱（吗啡、可待因、蒂巴因、罂粟碱和那可丁）。为此，开发并验证了一种基于固液萃取（SLE）的快速、简便且高效的方法，该方法在使用二极管阵列检测器的高效液相色谱（HPLC-DAD）分析之前进行。热降解研究表明，烘焙（180℃，20分钟）后托烷生物碱和鸦片生物碱的含量降低，阿托品的降解率在7%-65%之间，具体取决于所使用的制备条件；东莨菪碱和山莨菪碱的降解率在35%-49%之间；吗啡和可待因的降解率高达100%；蒂巴因、罂粟碱和那可丁的降解率在14%-58%之间。结果还表明，当将种子作为配料添加到面包棒上时，吗啡和可待因的降解率更高。
