Zhang Zhen, Hao Zhongna, Chai Rongyao, Qiu Haiping, Wang Yanli, Wang Jiaoyu, Sun Guochang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
J Fungi (Basel). 2022 Jul 26;8(8):780. doi: 10.3390/jof8080780.
Purines are basic components of nucleotides in living organisms. In this study, we identified the ortholog of adenylosuccinate synthase in by screening for growth-defective T-DNA insertional mutants. Gene replacement was performed to investigate the biological role of . mutants were adenine auxotrophs that failed to produce conidia, and showed reduced perithecia formation and pathogenicity. Moreover, the mutant was hypersensitive to Congo red and oxidants, indicating that was required for cell wall integrity and oxidative stress resistance. Transcriptomic analysis identified the underlying mechanisms and indicated that several pathogenicity-related genes were regulated in the mutant. Therefore, our data suggest that the adenylosuccinate synthase is involved in the de novo AMP biosynthesis pathway and is important for conidiation and pathogenicity in the rice blast fungus.
嘌呤是生物体内核苷酸的基本组成部分。在本研究中，我们通过筛选生长缺陷型T-DNA插入突变体，鉴定了[具体物种]中腺苷酸琥珀酸合酶的直系同源物。进行基因替换以研究[该基因]的生物学作用。[该基因]突变体是腺嘌呤营养缺陷型，无法产生分生孢子，并且子囊壳形成和致病性降低。此外，[该基因]突变体对刚果红和氧化剂高度敏感，表明[该基因]是细胞壁完整性和氧化应激抗性所必需的。转录组分析确定了潜在机制，并表明在[该基因]突变体中几个与致病性相关的基因受到调控。因此，我们的数据表明腺苷酸琥珀酸合酶[该基因]参与了从头合成AMP的生物合成途径，并且对稻瘟病菌的分生孢子形成和致病性很重要。
