Zhu Xueming, Li Lin, Wang Jiaoyu, Zhao Lili, Shi Huanbin, Bao Jiandong, Su Zhenzhu, Liu Xiaohong, Lin Fucheng
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311499, China.
J Fungi (Basel). 2021 Dec 17;7(12):1084. doi: 10.3390/jof7121084.
(synonym ) is a filamentous fungal pathogen that causes major yield losses in cultivated rice worldwide. However, the mechanisms of infection of are not well characterized. The VPS13 proteins play vital roles in various biological processes in many eukaryotic organisms, including in the organization of actin cytoskeleton, vesicle trafficking, mitochondrial fusion, and phagocytosis. Nevertheless, the function of the Vps13 protein in plant pathogenic fungi has not been explored. Here, we analysed the biological functions of the Vps13 protein in the development and pathogenicity of . Deletion mutants of MoVps13 significantly reduced the conidiation and decreased the rate of fungal infection on hosts. Moreover, the loss of MoVps13 resulted in defective cell wall integrity (CWI) and plasma membrane (PM) homeostasis when treated with chemicals for inducing cell wall stress (200 mg/mL Congo Red or 0.005% SDS) and sphingolipid synthesis inhibitors (2 μM myriocin or 2 μM amphotericin B). This indicated that MoVps13 is also involved in cell wall synthesis and sphingolipid synthesis. Through immunoblotting, autophagic flux detection, co-localization, and chemical drug sensitivity assays, we confirmed the involvement of Movps13 in ER-phagy and the response to ER stress. Additionally, we generated the C-terminal structure of MoVps13 with high accuracy using the alphaflod2 database. Our experimental evidence indicates that MoVps13 is an important virulence factor that regulates the pathogenicity of by controlling CWI, lipid metabolism and the ER-phagy pathway. These results have expanded our knowledge about pathogenic fungi and will help exploration for novel therapeutic strategies against the rice blast fungus.
（同义词）是一种丝状真菌病原体，在全球范围内导致栽培水稻大幅减产。然而，其感染机制尚未得到充分表征。VPS13蛋白在许多真核生物的各种生物过程中发挥着至关重要的作用，包括肌动蛋白细胞骨架的组织、囊泡运输、线粒体融合和吞噬作用。然而，Vps13蛋白在植物病原真菌中的功能尚未被探索。在此，我们分析了Vps13蛋白在（此处原文缺失相关真菌名称）发育和致病性中的生物学功能。MoVps13的缺失突变体显著降低了分生孢子形成，并降低了真菌对宿主的感染率。此外，当用诱导细胞壁应激的化学物质（200 mg/mL刚果红或0.005% SDS）和鞘脂合成抑制剂（2 μM肉豆蔻素或2 μM两性霉素B）处理时，MoVps13的缺失导致细胞壁完整性（CWI）和质膜（PM）稳态缺陷。这表明MoVps13也参与细胞壁合成和鞘脂合成。通过免疫印迹、自噬通量检测、共定位和化学药物敏感性分析，我们证实了Movps13参与内质网自噬和对内质网应激的反应。此外，我们使用alphaflod2数据库高精度生成了MoVps13的C端结构。我们的实验证据表明，MoVps13是一种重要的毒力因子，通过控制CWI、脂质代谢和内质网自噬途径来调节（此处原文缺失相关真菌名称）的致病性。这些结果扩展了我们对病原真菌的认识，并将有助于探索针对稻瘟病菌的新型治疗策略。
