Fraser Karl, Ryan Leigh, Dilger Ryan N, Dunstan Kelly, Armstrong Kelly, Peters Jason, Stirrat Hedley, Haggerty Neill, MacGibbon Alastair K H, Dekker James, Young Wayne, Roy Nicole C
AgResearch Ltd., Grasslands Research Centre, Palmerston North 4442, New Zealand.
Riddet Institute, Massey University, Palmerston North 4442, New Zealand.
Metabolites. 2022 Jul 26;12(8):689. doi: 10.3390/metabo12080689.
The milk fat globule membrane (MFGM) appears to play an important role in infant neurocognitive development; however, its mechanism(s) of action remains unclear. This study aimed to investigate the role of a dietary MFGM supplement on the lipid profiles of different neonatal brain regions. Ten-day-old male piglets (4−5 kg) were fed unsupplemented infant formula (control, n = 7) or an infant formula supplemented with low (4%) or high (8%) levels of MFGM (n = 8 each) daily for 21 days. Piglets were then euthanized, and brain tissues were sectioned. Untargeted liquid chromatography-mass spectrometry lipidomics was performed on the cerebellum, hippocampus, prefrontal cortex, and the rest of the brain. The analyses identified 271 and 171 lipids using positive and negative ionization modes, respectively, spanning 16 different lipid classes. MFGM consumption did not significantly alter the lipidome in most brain regions, regardless of dose, compared to the control infant formula. However, 16 triacylglyceride species were increased in the hippocampus (t-test, p-value < 0.05) of the high-supplemented piglets. Most lipids (262 (96.7%) and 160 (93.6%), respectively) differed significantly between different brain regions (ANOVA, false discovery rate corrected p-value < 0.05) independent of diet. Thus, this study highlighted that dietary MFGM altered lipid abundance in the hippocampus and detected large differences in lipid profiles between neonatal piglet brain regions.
乳脂肪球膜（MFGM）似乎在婴儿神经认知发育中发挥着重要作用；然而，其作用机制仍不清楚。本研究旨在探讨膳食补充MFGM对不同新生脑区脂质谱的影响。10日龄雄性仔猪（4 - 5千克）连续21天每天喂食未添加MFGM的婴儿配方奶粉（对照组，n = 7）或添加低水平（4%）或高水平（8%）MFGM的婴儿配方奶粉（每组n = 8）。然后对仔猪实施安乐死，并对脑组织进行切片。采用非靶向液相色谱 - 质谱脂质组学方法对小脑、海马体、前额叶皮质和大脑其他区域进行分析。在正离子和负离子电离模式下分别鉴定出271种和171种脂质，涵盖16种不同的脂质类别。与对照婴儿配方奶粉相比，无论剂量如何，食用MFGM在大多数脑区并未显著改变脂质组。然而，高剂量补充组仔猪海马体中的16种甘油三酯种类有所增加（t检验，p值<0.05）。大多数脂质（分别为262种（96.7%）和160种（93.6%））在不同脑区之间存在显著差异（方差分析，错误发现率校正p值<0.05），与饮食无关。因此，本研究强调膳食MFGM改变了海马体中的脂质丰度，并检测到新生仔猪脑区之间脂质谱存在巨大差异。
