Fernández-Fernández Rosa, Lozano Carmen, Ruiz-Ripa Laura, Robredo Beatriz, Azcona-Gutiérrez José Manuel, Alonso Carla Andrea, Aspiroz Carmen, Zarazaga Myriam, Torres Carmen
Área Bioquímica y Biología Molecular, OneHealth-UR Research Group, Universidad de La Rioja, 26006 Logroño, Spain.
Área Didáctica de las Ciencias Experimentales, OneHealth-UR Research Group, Universidad de La Rioja, 26006 Logroño, Spain.
Microorganisms. 2022 Jul 22;10(8):1480. doi: 10.3390/microorganisms10081480.
is a coagulase-negative-staphylococci (CoNS) that lately has gained special attention in public health as a human pathogen and also as a bacteriocin-producer bacteria. In this study, we characterized 56 isolates recovered from human samples in two Spanish hospitals. Antimicrobial susceptibility testing was performed and antimicrobial resistance and virulence genotypes were determined. Antimicrobial activity (AA) production was evaluated by the method against 37 indicator bacteria, including multidrug-resistant (MDR) isolates, and the presence of the D gene coding for lugdunin bacteriocin was analyzed by PCR. The antibiotic resistance detected was as follows (% resistance/genes detected): penicillin (44.6%/Z), oxacillin (1.8%/ on SCC-V), erythromycin-clindamycin inducible (7.1%/(C), ), tetracycline (5.3%/), gentamicin and/or tobramycin (3.6%/(4')-Ia, (6')-(2″)), and fosfomycin (21.4%). A MDR phenotype was detected in 5% of isolates. Twenty-one of the isolates showed susceptibility to all 20 antibiotics tested (37.5%). The screening for AA revealed 23 antimicrobial producer (AP) isolates with relevant inhibition against coagulase-positive-staphylococci (CoPS), including both methicillin-susceptible and -resistant . The D gene was detected in 84% of the 56 isolates. All of the AP isolates ( = 23) carried the D gene and it was also detected in 24 of the non-AP isolates, suggesting different gene expression levels. One of the AP isolates stood out due to its high antimicrobial activity against more than 70% of the indicator bacteria tested, so it will be further characterized at genomic and proteomic level.
是一种凝固酶阴性葡萄球菌（CoNS），最近作为一种人类病原体以及一种细菌素产生菌在公共卫生领域受到了特别关注。在本研究中，我们对从两家西班牙医院的人类样本中分离出的56株菌株进行了特征分析。进行了抗菌药敏试验，并确定了抗菌耐药性和毒力基因型。通过该方法对37种指示菌（包括多重耐药（MDR）菌株）评估了抗菌活性（AA）的产生，并通过PCR分析了编码路邓菌素细菌素的D基因的存在情况。检测到的抗生素耐药情况如下（耐药百分比/检测到的基因）：青霉素（44.6%/Z）、苯唑西林（1.8%/位于SCC-V上）、红霉素-克林霉素诱导型（7.1%/（C））、四环素（5.3%/）、庆大霉素和/或妥布霉素（3.6%/（4'）-Ia，（6'）-（2″））以及磷霉素（21.4%）。在5%的分离株中检测到了MDR表型。56株分离株中有21株对所有20种测试抗生素敏感（37.5%）。对AA的筛选发现了23株具有抗菌活性的分离株（AP），它们对凝固酶阳性葡萄球菌（CoPS）有显著抑制作用，包括甲氧西林敏感和耐药的菌株。在56株分离株中有84%检测到了D基因。所有AP分离株（n = 23）都携带D基因，在24株非AP分离株中也检测到了该基因，这表明基因表达水平不同。其中一株AP分离株因其对超过70%的测试指示菌具有高抗菌活性而脱颖而出，因此将在基因组和蛋白质组水平上进一步进行特征分析。
