Yan Lei, Liang Bin, Qi Meng-Yuan, Wang Ai-Jie, Liu Zhi-Pei
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Microorganisms. 2022 Jul 25;10(8):1496. doi: 10.3390/microorganisms10081496.
3-amino-5-methyl-isoxazole (3A5MI) is a persistent and harmful intermediate in the degradation of antibiotic sulfamethoxazole. It was accumulated in the environments day by day and has caused great environmental risks due to its refractory characteristic. Microbial degradation is economic and environmentally friendly and a promising method to eliminate this pollutant. In this study, a bacterial strain, sp. N39, was isolated. N39 can grow on 3A5MI as the sole carbon, nitrogen and energy resource. The effect of different factors on 3A5MI degradation by N39 was explored, including initial 3A5MI concentration, temperature, pH value, dissolved oxygen and additional carbon or nitrogen source. The degradation ability of N39 to various 3A5MI analogs was also explored. Nevertheless, the degrading ability of N39 for 3A5MI is not permanent, and long-term storage would lead to the loss of this ability. This may result from the mobile genetic elements in the bacterium according to the genomic comparison of N39 and its degrading ability-lost strain, N40. Despite this, N39 could support a lot of useful information about the degradation of 3A5MI and highlight the importance of studies about the environmental effects and potential degradation mechanism.
3-氨基-5-甲基异恶唑（3A5MI）是抗生素磺胺甲恶唑降解过程中一种持久性有害中间体。它在环境中日益积累，因其难降解特性已造成巨大环境风险。微生物降解经济且环保，是消除这种污染物的一种有前景的方法。在本研究中，分离出了一株细菌，即N39菌株。N39能够以3A5MI作为唯一碳源、氮源和能源生长。探究了不同因素对N39降解3A5MI的影响，包括初始3A5MI浓度、温度、pH值、溶解氧以及额外的碳源或氮源。还探究了N39对各种3A5MI类似物的降解能力。然而，N39对3A5MI的降解能力并非持久不变，长期保存会导致这种能力丧失。根据N39及其降解能力丧失菌株N40的基因组比较，这可能是由细菌中的可移动遗传元件导致的。尽管如此，N39可为3A5MI的降解提供许多有用信息，并凸显关于其环境影响和潜在降解机制研究的重要性。
