State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Nutrients. 2022 Jul 26;14(15):3057. doi: 10.3390/nu14153057.
Evidence shows that effective nutritional intervention can prevent or mitigate the risk and morbidity of inflammatory bowel disease (IBD). Bovine milk extracellular vesicles (mEVs), a major bioactive constituent of milk, play an important role in maintaining intestinal health. The aims of this study were to assess the effects of mEV pre-supplementation on the colonic transcriptome and proteome in dextran sulphate sodium (DSS)-induced acute colitis, in order to understand the underlying molecular mechanisms of mEV protection against acute colitis. Our results revealed that dietary mEV supplementation alleviated the severity of acute colitis, as evidenced by the reduced disease activity index scores, histological damage, and infiltration of inflammatory cells. In addition, transcriptome profiling analysis found that oral mEVs significantly reduced the expression of pro-inflammatory cytokines (, , and ), chemokine ligands (, , , , and ) and chemokine receptors ( and ). Moreover, oral mEVs up-regulated 109 proteins and down-regulated 150 proteins in the DSS-induced murine model, which were involved in modulating amino acid metabolism and lipid metabolism. Collectively, this study might provide new insights for identifying potential targets for the therapeutic effects of mEVs on colitis.
证据表明，有效的营养干预可以预防或减轻炎症性肠病（IBD）的风险和发病。牛乳细胞外囊泡（mEVs）是乳中的主要生物活性成分，在维持肠道健康方面发挥着重要作用。本研究旨在评估 mEV 预补充对葡聚糖硫酸钠（DSS）诱导的急性结肠炎结肠转录组和蛋白质组的影响，以了解 mEV 对急性结肠炎保护的潜在分子机制。我们的研究结果表明，膳食 mEV 补充可减轻急性结肠炎的严重程度，这一点可通过降低疾病活动指数评分、组织学损伤和炎症细胞浸润来证明。此外，转录组分析发现，口服 mEV 可显著降低促炎细胞因子（、、和）、趋化因子配体（、、、、和）和趋化因子受体（和）的表达。此外，口服 mEV 还可上调 DSS 诱导的小鼠模型中的 109 种蛋白质和下调 150 种蛋白质，这些蛋白质参与调节氨基酸代谢和脂质代谢。总的来说，这项研究可能为鉴定 mEV 对结肠炎的治疗效果的潜在靶点提供新的见解。
