Rawlings J M, Lucas M L, Russell R I
Scand J Gastroenterol. 1987 Apr;22(3):377-84. doi: 10.3109/00365528709078608.
Attaching plastic pH electrodes to a Crosby capsule provides a means of measuring mucosal surface pH in the small intestine during jejunal biopsy. Plastic electrodes, with operating characteristics comparable to glass electrodes within the physiological pH range, were selected because of their mechanical flexibility. The sensing tip can be placed close to the capsule aperture to facilitate contact with the intestinal mucosa. Using this system, the jejunal mucosal surface pH was found to be 5.97 +/- 0.07 in 15 control subjects. In contrast, 13 coeliac patients had a significantly (p less than 0.001) higher jejunal surface pH of 6.73 +/- 0.15, subdivisible into 4 untreated patients with a mean value of 6.95 +/- 0.33 and 9 patients on a gluten-free diet with a mean value of 6.63 +/- 0.15, both higher (p less than 0.01, p less than 0.005) than the control value. An elevated jejunal surface pH in coeliac disease was associated with partial or subtotal villous atrophy and with low brush border enzyme activities. Confirming previous in vitro findings, the elevated surface pH in coeliac jejunum may affect digestive and absorptive processes occurring at the brush border.
将塑料pH电极连接到克罗斯比胶囊上，提供了一种在空肠活检期间测量小肠粘膜表面pH值的方法。由于其机械柔韧性，选择了在生理pH范围内操作特性与玻璃电极相当的塑料电极。传感尖端可放置在靠近胶囊孔的位置，便于与肠粘膜接触。使用该系统，15名对照受试者的空肠粘膜表面pH值为5.97±0.07。相比之下，13名乳糜泻患者的空肠表面pH值显著升高（p<0.001），为6.73±0.15，可细分为4名未治疗患者，平均值为6.95±0.33，以及9名接受无麸质饮食的患者，平均值为6.63±0.15，两者均高于对照值（p<0.01，p<0.005）。乳糜泻患者空肠表面pH值升高与部分或全部绒毛萎缩以及刷状缘酶活性降低有关。证实了先前的体外研究结果，乳糜泻空肠中升高的表面pH值可能会影响在刷状缘发生的消化和吸收过程。
