Preclinical Research Unit, Drug Research Division, Sumitomo Pharma Co. Ltd, 3-1-98 Kasugade-naka, Konohana-ku, Osaka, 554-0022, Japan.
Doc Ophthalmol. 2022 Oct;145(2):147-155. doi: 10.1007/s10633-022-09890-y. Epub 2022 Jul 27.
We studied the conditions under which c-waves of the electroretinogram (ERG), that represent retinal pigment epithelium (RPE) function, were detectable using an alternating current (AC) amplifier and whether the c-wave recorded using an AC amplifier was useful for evaluating RPE function.
We recorded ERG responses in rats to 5 s stimuli under the conditions in which the low-cut frequency and the stimulus luminance were varied. In addition, changes in ERGs were studied after intravenous injection of sodium iodate (SI) to induce RPE degeneration.
The c-wave was detected clearly when the frequency of the low-cut filter was set at 0.01 Hz and light stimulus luminances were ≥ - 1.0 log cd/m. The c-wave was attenuated earlier than other waves (e.g., a-wave and b-wave) after SI administration.
The c-wave was easily detectable using an AC amplifier with the low-cut filter set at 0.01 Hz. Using the AC amplifier may allow easier c-wave recording, compared with the conventional use of a direct current (DC) amplifier, and could be useful for evaluating RPE function.
我们研究了在使用交流（AC）放大器的情况下，视网膜色素上皮（RPE）功能的视网膜电图（ERG）中的 c 波可检测到的条件，以及使用 AC 放大器记录的 c 波是否有助于评估 RPE 功能。
我们记录了大鼠在低频截止频率和刺激亮度变化的条件下对 5 s 刺激的 ERG 反应。此外，还研究了静脉注射碘酸钠（SI）诱导 RPE 变性后 ERG 的变化。
当低频截止滤波器的频率设置为 0.01 Hz 且光刺激亮度≥-1.0 log cd/m 时，可清晰检测到 c 波。在给予 SI 后，c 波比其他波（例如 a 波和 b 波）更早衰减。
使用低频截止滤波器设置为 0.01 Hz 的 AC 放大器可以轻松检测到 c 波。与传统使用直流（DC）放大器相比，使用 AC 放大器可能更容易记录 c 波，并有助于评估 RPE 功能。
