Jo Yonghyeon, Lee Ye-Ryoung, Hong Jin Hee, Kim Dong-Young, Kwon Junhwan, Choi Myunghwan, Kim Moonseok, Choi Wonshik
Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science, Seoul 02841, Republic of Korea.
Department of Physics, Korea University, Seoul 02855, Republic of Korea.
Sci Adv. 2022 Jul 29;8(30):eabo4366. doi: 10.1126/sciadv.abo4366. Epub 2022 Jul 27.
Compensation of sample-induced optical aberrations is crucial for visualizing microscopic structures deep within biological tissues. However, strong multiple scattering poses a fundamental limitation for identifying and correcting the tissue-induced aberrations. Here, we introduce a label-free deep-tissue imaging technique termed dimensionality reduction adaptive-optical microscopy (DReAM) to selectively attenuate multiple scattering. We established a theoretical framework in which dimensionality reduction of a time-gated reflection matrix can attenuate uncorrelated multiple scattering while retaining a single-scattering signal with a strong wave correlation, irrespective of sample-induced aberrations. We performed mouse brain imaging in vivo through the intact skull with the probe beam at visible wavelengths. Despite the strong scattering and aberrations, DReAM offered a 17-fold enhancement of single scattering-to-multiple scattering ratio and provided high-contrast images of neural fibers in the brain cortex with the diffraction-limited spatial resolution of 412 nanometers and a 33-fold enhanced Strehl ratio.
补偿样品引起的光学像差对于可视化生物组织深处的微观结构至关重要。然而，强烈的多重散射对识别和校正组织引起的像差构成了根本限制。在此，我们引入一种无标记的深层组织成像技术，称为降维自适应光学显微镜（DReAM），以选择性地减弱多重散射。我们建立了一个理论框架，其中时间选通反射矩阵的降维可以减弱不相关的多重散射，同时保留具有强波相关性的单次散射信号，而与样品引起的像差无关。我们通过完整的颅骨对小鼠大脑进行了体内成像，并将探测光束设置在可见波长。尽管存在强烈的散射和像差，DReAM仍使单次散射与多重散射的比率提高了17倍，并以412纳米的衍射极限空间分辨率提供了大脑皮层神经纤维的高对比度图像，且斯特列尔比提高了33倍。
