Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science, Seoul, 02841, Korea.
Department of Physics, Korea University, Seoul, 02841, Korea.
Nat Commun. 2019 Jul 17;10(1):3152. doi: 10.1038/s41467-019-11040-z.
Label-free in vivo imaging is crucial for elucidating the underlying mechanisms of many important biological systems in their most native states. However, the applicability of existing modalities has been limited to either superficial layers or early developmental stages due to tissue turbidity. Here, we report a synchronous angular scanning microscope for the rapid interferometric recording of the time-gated reflection matrix, which is a unique matrix characterizing full light-specimen interaction. By applying single scattering accumulation algorithm to the recorded matrix, we removed both high-order sample-induced aberrations and multiple scattering noise with the effective aberration correction speed of 10,000 modes/s. We demonstrated in vivo imaging of whole neural network throughout the hindbrain of the larval zebrafish at a matured stage where physical dissection used to be required for conventional imaging. Our method will expand the scope of applications for optical imaging, where fully non-invasive interrogation of living specimens is critical.
无标记活体成像是阐明许多重要生物系统在其自然状态下的潜在机制的关键。然而，由于组织浑浊，现有的模态的适用性仅限于浅层或早期发育阶段。在这里，我们报告了一种用于快速干涉记录时门控反射矩阵的同步角扫描显微镜，该矩阵是一种独特的矩阵，可表征全光与样本的相互作用。通过将单散射累积算法应用于记录的矩阵，我们去除了高阶样品诱导的像差和多次散射噪声，其有效像差校正速度为 10000 模式/秒。我们在成熟的斑马鱼幼虫的后脑中进行了整个神经网络的活体成像，而传统的成像方法需要进行物理解剖。我们的方法将扩展光学成像的应用范围，在这些应用中，对活体标本进行完全非侵入性的探测至关重要。
