Klämfeldt A, Marklund S
Scand J Rheumatol. 1987;16(1):41-5.
We have previously reported that addition of conditioned medium derived from fragment cultures of synovial tissue dissected from bovine knee-joints (SM) to cultures of articular cartilage derived from the same knee-joint resulted in enhanced breakdown of cartilage proteoglycans, measured as the release of [35S]sulphate from pieces of prelabelled cartilage. It has also been reported that oxygen-centered free radicals can degrade cartilage proteoglycans and that superoxide dismutase and catalase can reduce such degradation. Here we report that superoxide dismutase and catalase do not significantly affect the degradation of articular cartilage proteoglycans enhanced by conditioned synovial medium. It therefore appears that oxygen-centered free radicals are of at most minor importance for the degradation of cartilage enhanced by conditioned synovial medium.
我们之前曾报道，将从牛膝关节滑膜组织碎片培养物中获得的条件培养基（SM）添加到来自同一膝关节的关节软骨培养物中，会导致软骨蛋白聚糖的分解增强，这通过预先标记的软骨碎片中[35S]硫酸盐的释放来衡量。也有报道称，以氧为中心的自由基可降解软骨蛋白聚糖，超氧化物歧化酶和过氧化氢酶可减少这种降解。在此我们报告，超氧化物歧化酶和过氧化氢酶对经滑膜条件培养基增强的关节软骨蛋白聚糖降解没有显著影响。因此，以氧为中心的自由基对于经滑膜条件培养基增强的软骨降解似乎至多只起次要作用。
