Sugeçti Serkan, Tunçsoy Benay, Büyükgüzel Ender, Özalp Pınar, Büyükgüzel Kemal
Department of Veterinary Medicine, Çaycuma Food and Agriculture Vocational School, Zonguldak Bülent Ecevit University, Zonguldak, Turkey.
Faculty of Engineering, Department of Bioengineering, Adana Alparslan Turkeş Science and Technology University, Adana, Turkey.
J Environ Sci Health C Toxicol Carcinog. 2021;39(4):423-434. doi: 10.1080/26896583.2021.1969846. Epub 2021 Sep 9.
Nanoparticles (NPs) are now being used in many industrial activities, such as mining, paint and glass industries. The frequent industrial use of NPs contributes to environmental pollution and may cause cellular and oxidative damage in native organisms. In this study, the toxic effects of titanium dioxide nanoparticles (TiO NPs) were investigated using larvae as a model insect species. Alterations in cell damage indicators, such as alanine transferase, aspartate transferase, lactate dehydrogenase, non-enzymatic antioxidants and biochemical parameters, were determined in the hemolymph of larvae exposed to TiO NPs at different concentrations (5, 50, 250 and 1250 μg/mL) in their diets. TiO NPs caused concentration-dependent cellular damage in the hemolymph of larvae and increased the levels of the non-enzymatic antioxidants uric acid and bilirubin. In addition, total protein in hemolymph significantly decreased at the highest concentration (1250 μg/mL) of TiO NPs. Level of the urea increased at the highest concentration (1250 μg/mL) of TiO NPs, whereas the amount of glucose was not affected. These findings demonstrated that TiO NPs caused concentration-dependent toxic effects on larvae.
纳米颗粒（NPs）目前正被用于许多工业活动中，如采矿、涂料和玻璃行业。纳米颗粒在工业中的频繁使用导致了环境污染，并可能对原生生物造成细胞和氧化损伤。在本研究中，以幼虫作为模式昆虫物种，研究了二氧化钛纳米颗粒（TiO NPs）的毒性作用。在幼虫的食物中添加不同浓度（5、50、250和1250μg/mL）的TiO NPs，测定其血淋巴中细胞损伤指标（如丙氨酸转氨酶、天冬氨酸转氨酶、乳酸脱氢酶、非酶抗氧化剂）和生化参数的变化。TiO NPs在幼虫血淋巴中引起浓度依赖性细胞损伤，并增加了非酶抗氧化剂尿酸和胆红素的水平。此外，在TiO NPs最高浓度（1250μg/mL）时，血淋巴中的总蛋白显著降低。在TiO NPs最高浓度（1250μg/mL）时，尿素水平升高，而葡萄糖含量不受影响。这些发现表明，TiO NPs对幼虫具有浓度依赖性毒性作用。
