Institute of Hepatology, National Clinical Research Center for Infectious Disease, The Third People's Hospital of Shenzhen, The Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China.
Clinical Laboratory, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.
Front Immunol. 2022 Jul 8;13:880154. doi: 10.3389/fimmu.2022.880154. eCollection 2022.
Molecular assays on nasopharyngeal swabs act as a confirmatory test in coronavirus disease (COVID-19) diagnosis. However, the technical requirements of nasopharyngeal sampling and molecular assays limit the testing capabilities. Recent studies suggest the use of saliva for the COVID-19 diagnostic test. In this study, 44 patients diagnosed with COVID-19 in The Third People's Hospital of Shenzhen were enrolled. Saliva and serum specimens were obtained at different time points and the immunoglobulins against SARS-CoV-2 were measured. The results showed that saliva IgA presented a higher COI value than IgG and IgM. In matched saliva and serum samples, all saliva samples presented lower IgG levels than serum samples, and only one saliva sample presented a higher IgM level. The conversion rates of saliva IgA and the detection of viral nucleic acids were analyzed in the first and second weeks after hospitalization. The positive rates increased when combining saliva IgA and viral nucleic acid detection. In conclusion, our results provide evidence that saliva IgA could serve as a useful index for the early diagnosis of COVID-19.
对鼻咽拭子进行分子检测可作为冠状病毒病 (COVID-19) 诊断的确认试验。然而，鼻咽采样和分子检测的技术要求限制了检测能力。最近的研究表明，唾液可用于 COVID-19 的诊断检测。在这项研究中，共纳入了在深圳市第三人民医院确诊的 44 例 COVID-19 患者。在不同时间点采集唾液和血清标本，并检测针对 SARS-CoV-2 的免疫球蛋白。结果显示，唾液 IgA 的 COI 值高于 IgG 和 IgM。在配对的唾液和血清样本中，所有唾液样本的 IgG 水平均低于血清样本，只有一份唾液样本的 IgM 水平较高。分析了住院后第一周和第二周时唾液 IgA 的转化率和病毒核酸的检测情况。当结合唾液 IgA 和病毒核酸检测时，阳性率增加。总之，我们的结果为唾液 IgA 可作为 COVID-19 早期诊断的有用指标提供了证据。
