Li Bo, Wang Hui-Ying, Huang Jia-Hui, Xu Wan-Feng, Feng Xiao-Jie, Xiong Ze-Ping, Dong Ying-Jie, Li Lin-Zi, He Xinglishang, Wu Han-Song, Zhang Ke, Su Jie, Yu Qiao-Xian, Jiang Ning-Hua, Lv Gui-Yuan, Chen Su-Hong
Zhejiang University of Technology, Hangzhou, China.
Zhejiang Senyu Co., Ltd, Yiwu, China.
Front Pharmacol. 2022 Jul 11;13:935714. doi: 10.3389/fphar.2022.935714. eCollection 2022.
Metabolic hypertension (MH) is the most common type of hypertension worldwide because of unhealthy lifestyles, such as excessive alcohol intake and high-sugar/high-fat diets (ACHSFDs), adopted by humans. Poor diets lead to a decrease in the synthesis of short-chain fatty acids (SCFAs), which are produced by intestinal flora and transferred by G protein-coupled receptors (GPCRs), resulting in impaired gastrointestinal function, disrupted metabolic processes, increased blood pressure (BP), and ultimately, MH. It is not clear whether polysaccharide (DOPS) can mediate its effects by triggering the SCFAs-GPCR43/41 pathway. In this study, DOPS, with a content of 54.45 ± 4.23% and composition of mannose, glucose, and galacturonic acid at mass percentages of 61.28, 31.87, and 2.53%, was isolated from . It was observed that DOPS, given to rats by intragastric administration after dissolution, could lower the BP and improve the abnormal lipid metabolic processes in ACHSFD-induced MH rats. Moreover, DOPS was found to increase the production, transportation, and utilization of SCFAs, while improving the intestinal flora and strengthening the intestinal barrier, as well as increasing the intestinal levels of SCFAs and the expression of GPCR43/41. Furthermore, DOPS improved vascular endothelial function by increasing the expression of GPCR41 and endothelial nitric oxide synthase in the aorta and the nitric oxide level in the serum. However, these effects were all reversed by antibiotic use. These findings indicate that DOPS is the active component of , and it can reverse MH in rats by activating the intestinal SCFAs-GPCR43/41 pathway.
代谢性高血压（MH）是全球最常见的高血压类型，原因在于人类采取了不健康的生活方式，如过量饮酒和高糖/高脂饮食（ACHSFDs）。不良饮食导致短链脂肪酸（SCFAs）合成减少，SCFAs由肠道菌群产生并通过G蛋白偶联受体（GPCRs）传递，从而导致胃肠功能受损、代谢过程紊乱、血压（BP）升高，最终引发MH。目前尚不清楚多糖（DOPS）是否能通过触发SCFAs - GPCR43/41途径介导其作用。在本研究中，从……分离出了含量为54.45±4.23%、由甘露糖、葡萄糖和半乳糖醛酸组成且质量百分比分别为61.28%、31.87%和2.53%的DOPS。观察发现，溶解后经胃内给药给予大鼠的DOPS可降低ACHSFD诱导的MH大鼠的血压，并改善其异常脂质代谢过程。此外，发现DOPS可增加SCFAs的产生、运输和利用，同时改善肠道菌群、强化肠道屏障，以及提高肠道中SCFAs水平和GPCR43/41的表达。此外，DOPS通过增加主动脉中GPCR41和内皮型一氧化氮合酶的表达以及血清中一氧化氮水平来改善血管内皮功能。然而，这些作用都被抗生素使用所逆转。这些发现表明DOPS是……的活性成分，它可通过激活肠道SCFAs - GPCR43/41途径逆转大鼠的MH。
