Zhu Zhi-Han, Jia Feng, Ahmed Waqas, Zhang Gui-Long, Wang Hong, Lin Chao-Qun, Chen Wang-Hao, Chen Lu-Kui
Department of Neurosurgery, School of Medicine, Southeast University, Nanjing, Jiangsu Province, China.
Department of Neurosurgery, Neuroscience Center, Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China.
Neural Regen Res. 2023 Feb;18(2):404-409. doi: 10.4103/1673-5374.346466.
Our previous study demonstrated the potential therapeutic role of human neural stem cell-derived exosomes (hNSC-Exo) in ischemic stroke. Here, we loaded brain-derived neurotrophic factor (BDNF) into exosomes derived from NSCs to construct engineered exosomes (BDNF-hNSC-Exo) and compared their effects with those of hNSC-Exo on ischemic stroke both in vitro and in vivo. In a model of HO-induced oxidative stress in NSCs, BDNF-hNSC-Exo markedly enhanced cell survival. In a rat middle cerebral artery occlusion model, BDNF-hNSC-Exo not only inhibited the activation of microglia, but also promoted the differentiation of endogenous NSCs into neurons. These results suggest that BDNF can improve the function of NSC-derived exosomes in the treatment of ischemic stroke. Our research may support the clinical use of other neurotrophic factors for central nervous system diseases.
我们之前的研究证明了人神经干细胞来源的外泌体（hNSC-Exo）在缺血性脑卒中中的潜在治疗作用。在此，我们将脑源性神经营养因子（BDNF）载入神经干细胞来源的外泌体中构建工程化外泌体（BDNF-hNSC-Exo），并在体外和体内将其与hNSC-Exo对缺血性脑卒中的作用进行比较。在HO诱导的神经干细胞氧化应激模型中，BDNF-hNSC-Exo显著提高了细胞存活率。在大鼠大脑中动脉闭塞模型中，BDNF-hNSC-Exo不仅抑制了小胶质细胞的激活，还促进了内源性神经干细胞向神经元的分化。这些结果表明，BDNF可改善神经干细胞来源外泌体在缺血性脑卒中治疗中的功能。我们的研究可能支持其他神经营养因子在中枢神经系统疾病临床治疗中的应用。
