Sadeghi Sina, Pirsa Sajad, Asefi Narmela, Gharekhani Mehdi
Department of Food Science and Technology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran.
Sci Rep. 2025 Jul 2;15(1):23041. doi: 10.1038/s41598-025-07566-6.
In this study, cellulose nanofiber film was modified with methylene blue (MB) dye and vitamin C (Cel/MB/VC). The physicochemical and antibacterial characteristics of the prepared films were investigated. The rate of absorption and release of MB from the prepared film was studied. Films containing MB and VC were used as a kit to detect hydrogen peroxide. The results showed that MB and VC increased the thickness of the film and the elongation of the film. Both MB and VC agents reduced moisture and water vapor permeability, which significantly increased the antioxidant and antimicrobial properties (against Escherichia coli and Staphylococcus aureus) of the film. SEM images showed that the diameter of cellulose fibers is between 20 and 100 nm, which MB and VC completely cover their surface and the surface porosity of the film. FTIR spectra confirmed the electrostatic interactions between cellulose, MB and VC. According to the XRD results, cellulose has a crystalline structure, which is improved further on the vitamin C is added. According to TGA, MB and VC promoted thermal stability of the cellulose films. The release of methylene blue from the film was reported to be 15%. In the presence of HO, the color of the films containing MB and VC changed from white to blue, and that showed good performance as a hydrogen peroxide detection sensor (kit). The highest sensitivity of the sensor for measuring HO was 0.299 (100 mg/ml) with a detection limit of 1.9 (100 mg/ml).
在本研究中，用亚甲蓝（MB）染料和维生素C（Cel/MB/VC）对纤维素纳米纤维膜进行了改性。对制备的膜的物理化学和抗菌特性进行了研究。研究了MB从制备的膜中的吸收和释放速率。含MB和VC的膜被用作检测过氧化氢的试剂盒。结果表明，MB和VC增加了膜的厚度和膜的伸长率。MB和VC试剂均降低了水分和水蒸气渗透性，这显著提高了膜的抗氧化和抗菌性能（针对大肠杆菌和金黄色葡萄球菌）。扫描电子显微镜图像显示纤维素纤维的直径在20至100纳米之间，MB和VC完全覆盖了它们的表面以及膜的表面孔隙率。傅里叶变换红外光谱证实了纤维素、MB和VC之间的静电相互作用。根据X射线衍射结果，纤维素具有晶体结构，添加维生素C后进一步得到改善。根据热重分析，MB和VC提高了纤维素膜的热稳定性。据报道，膜中亚甲蓝的释放率为15%。在过氧化氢存在下，含MB和VC的膜的颜色从白色变为蓝色，这表明其作为过氧化氢检测传感器（试剂盒）具有良好的性能。该传感器测量过氧化氢的最高灵敏度为0.299（100毫克/毫升），检测限为1.9（100毫克/毫升）。
