基于多功能化氧化石墨烯的用于超灵敏检测糖类抗原 19-9 的一步式电化学发光免疫传感器的制备。
A one-step electrochemiluminescence immunosensor preparation for ultrasensitive detection of carbohydrate antigen 19-9 based on multi-functionalized graphene oxide.
机构信息
Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, PR China.
Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, PR China.
出版信息
Biosens Bioelectron. 2015 Apr 15;66:468-73. doi: 10.1016/j.bios.2014.12.013. Epub 2014 Dec 3.
A one-step electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of carbohydrate antigen 19-9 (CA19-9) was developed based on multi-functionalized graphene oxide (GO), which was prepared with N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and CA19-9 antibody (anti-CA19-9) chemically bound to the surface of magnetic GO (nanoFe3O4@GO). ABEI and anti-CA19-9 acted as the electrochemiluminophore and the capture device for CA19-9 respectively. NanoFe3O4@GO enabled all the ABEI immobilized molecules electrochemically active due to its good conductivity, and brought multi-functionalized GO attracted on the surface of magnetic glass carbon electrode through magnetism. Thus the ECL immunosensor could be prepared through a one-step process that facilitates ultrasensitive detection of CA19-9. Under optimal conditions, the ECL intensity of the immunosensor decreased proportionally to the logarithmic concentrations of CA19-9 in the range of 0.001-5U/mL with a detection limit of 0.0005U/mL. This one-step ECL immunosensor showed good performance in specificity, stability, reproducibility, regeneration and application. It opened a new avenue to apply multi-functionalized bionanomaterials in ECL immunoassay.
基于多功能化氧化石墨烯（GO），我们开发了一种用于超灵敏检测糖链抗原 19-9（CA19-9）的一步式电化学发光（ECL）免疫传感器。GO 是通过 N-(4-氨基丁基)-N-乙基异鲁米诺（ABEI）和 CA19-9 抗体（抗 CA19-9）化学结合到磁性 GO（nanoFe3O4@GO）表面而制备的。ABEI 和抗 CA19-9 分别作为电化学发光体和 CA19-9 的捕获装置。由于良好的导电性，nanoFe3O4@GO 使所有固定化的 ABEI 分子具有电化学活性，并通过磁性将多功能化的 GO 吸引到磁性玻碳电极的表面。因此，通过一步法可以制备 ECL 免疫传感器，从而实现 CA19-9 的超灵敏检测。在最佳条件下，该免疫传感器的 ECL 强度与 CA19-9 的对数浓度在 0.001-5U/mL 范围内呈比例下降，检测限为 0.0005U/mL。这种一步式 ECL 免疫传感器在特异性、稳定性、重现性、再生和应用方面表现出良好的性能。它为在 ECL 免疫分析中应用多功能生物纳米材料开辟了新途径。
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