Petraglia Tania, Latronico Tiziana, Pepe Antonietta, Crescenzi Aniello, Liuzzi Grazia Maria, Rossano Rocco
Department of Basic and Applied Sciences, University of Basilicata, 85100 Potenza, Italy.
Department of Biosciences, Biotechnologies and Environment, University of Bari "Aldo Moro", 70126 Bari, Italy.
Molecules. 2024 Nov 26;29(23):5575. doi: 10.3390/molecules29235575.
The aim of this study was to evaluate the antioxidant properties of the products derived from the biodegradation of lignin by the ligninolytic enzymes present in an aqueous extract of the mushroom . A mixture obtained after the incubation of lignin for 18 h with extract was tested in vitro for its total polyphenol content, reducing power, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl (OH) radical-scavenging activities. The results showed that the enzymatic treatment of lignin enhanced its antioxidant performance. The biocompatibility of the products of lignin biodegradation and their ability to scavenge reactive oxygen species (ROS) were also tested on the astrocytic cell line DI-TNC1. The results obtained indicated that a lignin mixture incubated for 18 h does not affect cell viability or inhibit the HO-induced ROS production. These results suggest that the enzymatic degradation of lignin represents an efficient and ecofriendly approach to obtain lignin derivatives potentially useful for antioxidant applications.
本研究的目的是评估蘑菇水提物中存在的木质素分解酶对木质素生物降解产物的抗氧化性能。将木质素与提取物孵育18小时后得到的混合物，在体外测试其总多酚含量、还原能力以及1,1-二苯基-2-苦基肼（DPPH）和羟基（OH）自由基清除活性。结果表明，对木质素进行酶处理可增强其抗氧化性能。还在星形胶质细胞系DI-TNC1上测试了木质素生物降解产物的生物相容性及其清除活性氧（ROS）的能力。所得结果表明，孵育18小时的木质素混合物不会影响细胞活力或抑制HO诱导的ROS产生。这些结果表明，木质素的酶促降解是一种高效且环保的方法，可获得可能用于抗氧化应用的木质素衍生物。
