Kawaka Fanuel
Department of Biological Sciences, Jaramogi Oginga Odinga University of Science and Technology, P.O. Box 210-40601, Bondo, Kenya.
AMB Express. 2022 Jul 30;12(1):99. doi: 10.1186/s13568-022-01441-7.
Symbiotic nitrogen fixing bacteria comprise of diverse species associated with the root nodules of leguminous plants. Using an appropriate taxonomic method to confirm the identity of superior and elite strains to fix nitrogen in legume crops can improve sustainable global food and nutrition security. The current review describes taxonomic methods preferred and commonly used to characterize symbiotic bacteria in the rhizosphere. Peer reviewed, published and unpublished articles on techniques used for detection, classification and identification of symbiotic bacteria were evaluated by exploring their advantages and limitations. The findings showed that phenotypic and cultural techniques are still affordable and remain the primary basis of species classification despite their challenges. Development of new, robust and informative taxonomic techniques has really improved characterization and identification of symbiotic bacteria and discovery of novel and new species that are effective in biological nitrogen fixation (BNF) in diverse conditions and environments.
共生固氮细菌由与豆科植物根瘤相关的多种物种组成。使用适当的分类方法来确认豆科作物中固氮的优良菌株的身份，可以改善全球可持续的粮食和营养安全。当前的综述描述了用于表征根际共生细菌的首选和常用分类方法。通过探讨其优点和局限性，对有关共生细菌检测、分类和鉴定技术的同行评审、已发表和未发表的文章进行了评估。研究结果表明，表型和培养技术仍然经济实惠，尽管存在挑战，但仍是物种分类的主要依据。新的、强大的和信息丰富的分类技术的发展确实改进了共生细菌的表征和鉴定，以及在不同条件和环境下对生物固氮（BNF）有效的新物种的发现。
