Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.
Int J Biol Macromol. 2022 Sep 30;217:969-978. doi: 10.1016/j.ijbiomac.2022.07.187. Epub 2022 Jul 28.
Amino functionalized chitosan has attracted much attention because of the fascinated bioactivities. In our study, a novel water-soluble amino functionalized chitosan bearing free amino group at C-2 and quaternary ammonium moiety contained free amino group at C-6 (5c) was prepared by a four-step method. The structural characterization was identified by FTIR and H NMR spectroscopy. The water-solubility and antioxidant activities against hydroxyl, DPPH radicals and reducing power were estimated. The results displayed that amino functionalized chitosan 5c exhibited improved water-solubility and antioxidant ability, especially its DPPH scavenging rate reached about 90 % at the minimum test concentration of 0.10 mg/mL. Besides, antibacterial tests showed that amino functional chitosan 5c had best antibacterial activities, which indicated that amino group made main contribution to the enhanced bioactivities. In short, the novel chitosan 5c possessed enhanced water-solubility and excellent antioxidant and antibacterial activities, which could provide novel strategy for the development of antioxidant and antibacterial agents in biomedicine and food fields.
由于迷人的生物活性，氨基功能化壳聚糖引起了广泛关注。在我们的研究中，通过四步反应法制备了一种新型的水溶性氨基功能化壳聚糖，其在 C-2 位上具有游离氨基，在 C-6 位上具有季铵盐基和游离氨基（5c）。通过傅里叶变换红外光谱（FTIR）和核磁共振氢谱（1H NMR）对其结构进行了表征。评估了其水溶性和对羟基自由基、DPPH 自由基和还原能力的抗氧化活性。结果表明，氨基功能化壳聚糖 5c 具有改善的水溶性和抗氧化能力，特别是在最低测试浓度为 0.10mg/mL 时，其 DPPH 清除率达到约 90%。此外，抑菌试验表明，氨基功能化壳聚糖 5c 具有最佳的抑菌活性，这表明氨基是增强生物活性的主要贡献者。总之，新型壳聚糖 5c 具有增强的水溶性和优异的抗氧化和抑菌活性，可为生物医学和食品领域抗氧化和抑菌剂的开发提供新策略。
