Distribution and clinical significance of circulating CD8CD28 regulatory T cells in the peripheral blood of patients with pulmonary tuberculosis.

BACKGROUND

Regulatory T cells (Treg cells) in the peripheral blood of patients with pulmonary tuberculosis (PTB) may be closely related to the progression of PTB. In this study, the distribution characteristics and clinical importance of CD8CD28 Treg cells in patients with tuberculosis were systematically analyzed, and the role and importance of CD8CD28 Treg cells in influencing the immune response and progression of tuberculosis were discussed, which will provide immunological indices and reference values for the clinical diagnosis of tuberculosis.

METHODS

Flow cytometry, sputum smears and computed tomography imaging were used to analyze the distribution characteristics of CD8CD28 Treg cells in the peripheral blood of patients with PTB and the correlation between CD8CD28Treg cells and clinical and immune indices.

RESULTS

The percentages of CD4CD25 and CD8CD28 Treg cells in the peripheral blood of patients with PTB were significantly higher than those in the healthy control (HC) group. Further analysis showed that the percentage of CD4CD25Treg cells in the Stage II group was significantly higher than that in the HC group. The percentages of CD4CD25 and CD8CD28 Treg cells increased significantly in patients in the Stage II group. The proportion of CD8CD28 Treg cells was directly proportional to the degree of positivity in sputum smears, while CD4CD25Treg cells did not exhibit this trend. The correlations between the percentage of CD4CD25 and CD8CD28 Treg cells and the percentage of lymphocyte subsets were examined. The percentage of CD8CD28 Treg cells was negatively correlated with the percentage of CD4T cells and positively correlated with the CD8T cell percentage in the HC and PTB groups. The percentage of CD4 + CD25Treg cells was positively correlated with the percentage of CD4T cells only in the PTB group.

CONCLUSIONS

This study was the first to show that the proportion of CD8CD28 Treg cells in the peripheral blood of patients with PTB was significantly increased, and the increase in CD8CD28 Treg cells was related to the progression of PTB, which may affect the proportion of immune cell subsets by inhibiting the immune response, resulting in the progression of PTB.