Department of Clinical Laboratory, The Fifth People's Hospital of Suzhou, 10, Guanqian Road, Suzhou, 215000, Jiangsu, People's Republic of China.
Department of Respiratory, The Affiliation Infections Diseases Hospital of Soochow University, 10 Guangqian Road, Suzhou, 215000, Jiangsu, People's Republic of China.
BMC Pulm Med. 2022 Jul 30;22(1):291. doi: 10.1186/s12890-022-02088-7.
Regulatory T cells (Treg cells) in the peripheral blood of patients with pulmonary tuberculosis (PTB) may be closely related to the progression of PTB. In this study, the distribution characteristics and clinical importance of CD8CD28 Treg cells in patients with tuberculosis were systematically analyzed, and the role and importance of CD8CD28 Treg cells in influencing the immune response and progression of tuberculosis were discussed, which will provide immunological indices and reference values for the clinical diagnosis of tuberculosis.
Flow cytometry, sputum smears and computed tomography imaging were used to analyze the distribution characteristics of CD8CD28 Treg cells in the peripheral blood of patients with PTB and the correlation between CD8CD28Treg cells and clinical and immune indices.
The percentages of CD4CD25 and CD8CD28 Treg cells in the peripheral blood of patients with PTB were significantly higher than those in the healthy control (HC) group. Further analysis showed that the percentage of CD4CD25Treg cells in the Stage II group was significantly higher than that in the HC group. The percentages of CD4CD25 and CD8CD28 Treg cells increased significantly in patients in the Stage II group. The proportion of CD8CD28 Treg cells was directly proportional to the degree of positivity in sputum smears, while CD4CD25Treg cells did not exhibit this trend. The correlations between the percentage of CD4CD25 and CD8CD28 Treg cells and the percentage of lymphocyte subsets were examined. The percentage of CD8CD28 Treg cells was negatively correlated with the percentage of CD4T cells and positively correlated with the CD8T cell percentage in the HC and PTB groups. The percentage of CD4 + CD25Treg cells was positively correlated with the percentage of CD4T cells only in the PTB group.
This study was the first to show that the proportion of CD8CD28 Treg cells in the peripheral blood of patients with PTB was significantly increased, and the increase in CD8CD28 Treg cells was related to the progression of PTB, which may affect the proportion of immune cell subsets by inhibiting the immune response, resulting in the progression of PTB.
肺结核（PTB）患者外周血中的调节性 T 细胞（Treg 细胞）可能与 PTB 的进展密切相关。本研究系统分析了结核患者中 CD8CD28Treg 细胞的分布特征及其临床意义，并探讨了 CD8CD28Treg 细胞在影响结核免疫反应和进展中的作用及重要性，为结核病的临床诊断提供免疫学指标及参考值。
采用流式细胞术、痰涂片及 CT 影像学分析 PTB 患者外周血中 CD8CD28Treg 细胞的分布特征及其与临床免疫指标的相关性。
PTB 患者外周血中 CD4CD25 和 CD8CD28Treg 细胞的比例明显高于健康对照组（HC 组）。进一步分析显示，Ⅱ期组 CD4CD25Treg 细胞的比例明显高于 HC 组。Ⅱ期组患者外周血中 CD4CD25 和 CD8CD28Treg 细胞的比例明显增高，且 CD8CD28Treg 细胞的比例与痰涂片阳性度呈正相关，而 CD4CD25Treg 细胞无此趋势。同时检测 CD4CD25 和 CD8CD28Treg 细胞与淋巴细胞亚群百分比的相关性，发现 CD8CD28Treg 细胞的比例与 HC 组和 PTB 组的 CD4T 细胞比例呈负相关，与 CD8T 细胞比例呈正相关，而 CD4CD25Treg 细胞仅在 PTB 组与 CD4T 细胞比例呈正相关。
本研究首次表明，PTB 患者外周血中 CD8CD28Treg 细胞的比例明显增加，且 CD8CD28Treg 细胞的增加与 PTB 的进展有关，可能通过抑制免疫反应影响免疫细胞亚群的比例，导致 PTB 的进展。
