Wu Min, Li Qiulin, Xia Guixian, Zhang Yongshan, Wang Fuxin
College of Life Sciences, Hebei University, Baoding 071002, China; and Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; and University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences, Anyang, Henan 455000, China.
Funct Plant Biol. 2022 Oct;49(11):980-994. doi: 10.1071/FP22006.
Verticillium wilt is a highly destructive fungal disease that attacks a broad range of plants, including many major crops. However, the mechanism underlying plant immunity toward Verticillium dahliae is very complex and requires further study. By combining bioinformatics analysis and experimental validation, we investigated plant defence responses against V. dahliae infection in the model plant Arabidopsis thaliana L. A total of 301 increased and 214 decreased differentially abundant proteins (DAPs) between mock and infected wild type (WT) plants were acquired and bioinformatics analyses were then conducted and compared (increased vs decreased) in detail. In addition to the currently known mechanisms, several new clues about plant immunity against V. dahliae infection were found in this study: (1) exosome formation was dramatically induced by V. dahliae attack; (2) tryptophan-derived camalexin and cyanogenic biosynthesis were durably promoted in response to infection; and (3) various newly identified components were activated for hub immunity responses. These new clues provide valuable information that extends the current knowledge about the molecular basis of plant immunity against V. dahliae infection.
黄萎病是一种极具破坏性的真菌病害，可侵袭多种植物，包括许多主要农作物。然而，植物对大丽轮枝菌的免疫机制非常复杂，需要进一步研究。通过结合生物信息学分析和实验验证，我们在模式植物拟南芥中研究了植物对大丽轮枝菌感染的防御反应。在 mock 和感染的野生型（WT）植物之间共获得了 301 种丰度增加和 214 种丰度降低的差异丰富蛋白（DAPs），然后进行了生物信息学分析并详细比较（增加与降低）。除了目前已知的机制外，本研究还发现了一些关于植物对大丽轮枝菌感染免疫的新线索：（1）大丽轮枝菌攻击显著诱导外泌体形成；（2）感染后，色氨酸衍生的植保素和氰化物生物合成持续增强；（3）多种新鉴定的成分被激活以产生核心免疫反应。这些新线索提供了有价值的信息，扩展了我们目前对植物抗大丽轮枝菌感染分子基础的认识。
