Bashir Asiya, Asif Muhammad, Saadullah Malik, Saleem Mohammad, Khalid Syed Haroon, Hussain Liaqat, Ullah Khan Ikram, Sidra Yaseen Hafiza, Zubair Hafiz Muhammad, Shamas Muhammad Usman, Al Zarzour Raghdaa, Chohan Tahir Ali
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, Pakistan.
Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, 63100 Punjab, Pakistan.
ACS Omega. 2022 Jul 15;7(29):25772-25782. doi: 10.1021/acsomega.2c03053. eCollection 2022 Jul 26.
(L.) All. is known to have anti-inflammatory and anticancer properties. The present study explored the skin carcinogenesis attenuating potential of ethanolic extract of (L.) All. (Miet) in a 7,12-dimethylbenz[]anthracene (DMBA)-induced skin cancer model. The ethanolic extract of the plant was prepared by a maceration method. HPLC analysis indicated the presence of quercetin in abundance and also various other phytoconstituents. DPPH radical scavenging assay results showed moderate antioxidant potential (IC = 93.55 ± 5.59 μg/mL). A topical acute skin irritation study showed the nonirritant nature of Miet. Data for the skin carcinogenic model showed marked improvement in skin architecture in Miet and its primary phytochemicals (quercetin and coumarin) treated groups. Miet 50% showed comparable effects with 5-fluorouracil. Significant ( < 0.05) anticancerous effects were seen in coumarin-quercetin combination-treated animals than in single agent (coumarin and quercetin alone)-treated animals. Chorioallantoic membrane (CAM) assay results showed the antiangiogenic potential of Miet. Treatment with Miet significantly down-regulated the serum levels of CEA (carcinoembryonic antigen) and TNF-α (Tumor necrosis factor-α). Data for the docking study indicated the binding potential of quercetin and coumarin with TNF-α, EGFR, VEGF, and BCL2 proteins. Thus, it is concluded that Miet has skin cancer attenuating potential that is proposed to be due to the synergistic actions of its bioactive molecules. Further studies to explore the effects of Miet and its bioactive molecules as an adjuvant therapy with low dose anticancer drugs are warranted, which may lead to a new area of research.
众所周知，（此处植物学名未完整给出，无法准确翻译，假设为某种植物名称）具有抗炎和抗癌特性。本研究在7,12 - 二甲基苯并[a]蒽（DMBA）诱导的皮肤癌模型中，探究了该植物乙醇提取物（Miet）减轻皮肤癌发生的潜力。该植物的乙醇提取物采用浸渍法制备。高效液相色谱分析表明，提取物中富含槲皮素以及其他多种植物成分。二苯基苦味酰基自由基清除试验结果显示其具有中等抗氧化潜力（IC = 93.55 ± 5.59 μg/mL）。局部急性皮肤刺激性研究表明Miet无刺激性。皮肤癌模型的数据显示，Miet及其主要植物化学成分（槲皮素和香豆素）处理组的皮肤结构有显著改善。50%的Miet表现出与5 - 氟尿嘧啶相当的效果。香豆素 - 槲皮素联合处理的动物比单药（单独的香豆素和槲皮素）处理的动物具有更显著（P < 0.05）的抗癌作用。鸡胚绒毛尿囊膜（CAM）试验结果显示Miet具有抗血管生成潜力。用Miet处理可显著下调癌胚抗原（CEA）和肿瘤坏死因子 - α（TNF - α）的血清水平。对接研究数据表明槲皮素和香豆素与TNF - α、表皮生长因子受体（EGFR）、血管内皮生长因子（VEGF）和B细胞淋巴瘤2（BCL2）蛋白具有结合潜力。因此，得出结论：Miet具有减轻皮肤癌的潜力，推测这归因于其生物活性分子之间的协同作用。有必要进一步研究探索Miet及其生物活性分子作为低剂量抗癌药物辅助治疗的效果，这可能会开创一个新的研究领域。
