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超声辅助绿色溶剂从微藻 Nannochloropsis spp. 中提取高附加值化合物

Ultrasound-assisted green solvent extraction of high-added value compounds from microalgae Nannochloropsis spp.

机构信息

Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, B.P. 20529, 60205 Compiègne Cedex, France; Institute of Biocolloidal Chemistry named after F. D. Ovcharenko, NAS of Ukraine, 42, Blvr. Vernadskogo, Kyiv 03142, Ukraine.

Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132 Fisciano (SA), Italy.

出版信息

Bioresour Technol. 2015 Dec;198:262-7. doi: 10.1016/j.biortech.2015.09.020. Epub 2015 Sep 15.

DOI:10.1016/j.biortech.2015.09.020

PMID:26398670

Abstract

The aim of this work was to investigate ultrasound (US)-assisted green solvent extraction of valuable compounds from the microalgae Nannochloropsis spp. Individual green solvents (water, ethanol (EtOH), dimethyl sulfoxide (DMSO)) and binary mixture of solvents (water-DMSO and water-EtOH) were used for the extraction procedures. Maximum total phenolic compounds yield (Yp ≈ 0.33) was obtained after US pre-treatment (W=400 W, 15 min), being almost 5-folds higher compared to that found for the untreated samples and aqueous extraction (Yp ≈ 0.06). The highest yield of total chlorophylls (Yc ≈ 0.043) was obtained after US (W=400 W, 7.5 min), being more than 9-folds higher than those obtained for the untreated samples and aqueous extraction (Yc ≈ 0.004). The recovery efficiency decreased as DMSO>EtOH>H2O. The optimal conditions to recover phenolic compounds and chlorophylls from microalgae were obtained after US pre-treatment (400 W, 5 min), binary mixtures of solvents (water-DMSO and water-EtOH) at 25-30%, and microalgae concentration of 10%.

摘要

本工作旨在研究超声（US）辅助从微藻中提取有价值化合物的绿色溶剂萃取法。采用单一绿色溶剂（水、乙醇（EtOH）、二甲基亚砜（DMSO））和溶剂二元混合物（水-DMSO 和水-EtOH）进行萃取操作。超声预处理（W=400 W，15 min）后，总酚类化合物的最大产率（Yp≈0.33），几乎比未处理样品和水萃取（Yp≈0.06）高 5 倍。超声（W=400 W，7.5 min）后，总叶绿素的最大产率（Yc≈0.043），比未处理样品和水萃取（Yc≈0.004）高 9 倍以上。DMSO>EtOH>H2O，回收率效率降低。从微藻中回收酚类化合物和叶绿素的最佳条件是超声预处理（400 W，5 min）、溶剂二元混合物（水-DMSO 和水-EtOH）在 25-30%，微藻浓度为 10%。