Miers M K, Fogo A B, Federspiel C F, McAllister N W, Phillips P A, Cousar J B
Am J Clin Pathol. 1987 Jun;87(6):745-51. doi: 10.1093/ajcp/87.6.745.
The Coulter S-Plus IV separates leukocytes in peripheral blood by volume analysis into granulocytes, lymphocytes, and mononuclear cell fractions, the so-called three-part differential (3PD). White blood cell (WBC) counts, hemoglobin concentrations, platelet counts, 3PDs, and histograms were compared with smear differentials on 3,861 samples, including samples from oncology patients, to determine the predictive value of the Coulter parameters in detecting smear abnormalities. Results showed good correlation between the 3PD and smear differential for granulocytes and lymphocytes (correlation coefficients were 0.883 and 0.868, respectively) and poor correlation for mononuclear cells (0.492). No histogram region flag indicators were observed in the following samples: 15 of 40 samples with greater than or 1% blasts; 105 of 183 with greater than or 1 nucleated red blood cell (NRBC) per 100 WBCs; 65 of 113 with monocytosis greater than 1,600/microL (1.6 X 10(9)/L); 425 of 548 with greater than 2,000 bands/microL (2.0 X 10(9)/L); 84 of 112 with eosinophilia greater than 600/microL (0.6 X 10(9)L); and 88 of 149 with greater than 2% immature granulocytes. All samples with greater than 5% blasts were indicated by region flags and abnormal histograms. Oncology patient data were analyzed, yielding similar results. The authors established guidelines for using the Coulter 3PD data in their tertiary care laboratory to select samples that require smear evaluation.
库尔特S-Plus IV型血细胞分析仪通过体积分析将外周血中的白细胞分离为粒细胞、淋巴细胞和单核细胞组分，即所谓的三分群分类法（3PD）。对3861份样本（包括肿瘤患者的样本）的白细胞（WBC）计数、血红蛋白浓度、血小板计数、3PD结果和直方图与涂片分类结果进行比较，以确定库尔特参数在检测涂片异常方面的预测价值。结果显示，粒细胞和淋巴细胞的3PD结果与涂片分类结果之间具有良好的相关性（相关系数分别为0.883和0.868），而单核细胞的相关性较差（0.492）。在以下样本中未观察到直方图区域标记指标：40份有大于或等于1%原始细胞的样本中的15份；183份每100个WBC中有大于或等于1个有核红细胞（NRBC）的样本中的105份；113份单核细胞增多大于1600/μL（1.6×10⁹/L）的样本中的65份；548份杆状核细胞大于2000/μL（2.0×10⁹/L）的样本中的425份；112份嗜酸性粒细胞大于600/μL（0.6×10⁹/L）的样本中的84份；以及149份未成熟粒细胞大于2%的样本中的88份。所有原始细胞大于5%的样本均通过区域标记和异常直方图显示。对肿瘤患者的数据进行分析，得出了类似的结果。作者制定了在其三级医疗实验室中使用库尔特3PD数据来选择需要涂片评估的样本的指导原则。
