Alidadi Soodeh, Nourani Hossein, Vakili Toraj, Hassanabadi Morteza
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Private Veterinary Practitioner, Aftab Pets Specialty Clinic, Mashhad, Iran.
Vet Res Forum. 2022 Jun;13(2):289-292. doi: 10.30466/vrf.2021.534469.3216. Epub 2022 Jun 15.
A 14-year- old female Shih Tzu was presented with conjunctivitis, keratitis, an enlarged mass located on the left lower eyelid and a large subcutaneous cyst in the temporal region. The hematological and biochemical analyses revealed no significant issues. The mass and the cyst were taken from the base with 1.50 cm of the underlying tissue. On histological examination, the mass was made up of adenoid arrangements of sebocytes and basaloid reserve cells with low to moderate mitotic activity of basaloid cells. In addition, a large number of meibomian ducts with marked infiltration of mononuclear inflammatory cells and melanin pigment were observed in the ductal part of the tumor. The cyst was lined by a simple cuboid-like epithelium with no eosinophilic cuticular lining. Immuno-histochemical staining of the mass with CK14 showed positive reaction in the neoplastic basaloid reserve cells and adipocytes; but, not with CK18 and vimentin. Unlike, the luminal cells of the cyst were stained with CK18. The co-existence of mixed meibomian gland and ductal adenoma with an apocrine sweat gland cyst was diagnosed based on the clinical, histological and immunohistochemical findings.
一只14岁雌性西施犬出现结膜炎、角膜炎，左下眼睑有一肿大肿物，颞部有一个大的皮下囊肿。血液学和生化分析未发现明显问题。肿物和囊肿从基部连同其下方1.50 cm的组织一并切除。组织学检查显示，肿物由皮脂腺细胞和基底样储备细胞的腺样排列组成，基底样细胞有低至中度的有丝分裂活性。此外，在肿瘤的导管部分观察到大量睑板腺导管，有明显的单核炎性细胞浸润和黑色素沉着。囊肿内衬单层立方形上皮，无嗜酸性角质层内衬。肿物用细胞角蛋白14（CK14）进行免疫组织化学染色，结果显示肿瘤性基底样储备细胞和脂肪细胞呈阳性反应；但细胞角蛋白18（CK18）和波形蛋白染色为阴性。与之不同的是，囊肿的腔面细胞CK18染色呈阳性。根据临床、组织学和免疫组织化学检查结果，诊断为睑板腺和导管混合性腺瘤与顶泌汗腺囊肿并存。
