Department of General Surgery, Hatay Mustafa Kemal University Faculty of Medicine, Hatay-Türkiye.
Department of Biology, Hatay Mustafa Kemal University Faculty of Arts and Science, Hatay-Türkiye.
Ulus Travma Acil Cerrahi Derg. 2022 Aug;28(8):1059-1065. doi: 10.14744/tjtes.2022.90838.
Failure to achieve effective bleeding control and problems related to transfusion in liver surgery are the most common causes of post-operative mortality and morbidity. Various methods/drugs including topical hemostatic agents have been em-ployed for bleeding control in liver surgery. This study was aimed to investigate the hemostatic properties of the herb mixture extract of Inula viscosa and Capsella bursa-pastoris (IvCbp) in rat liver laceration model, which have been traditionally used as antiseptic and hemostatic agents public in Hatay/Tukey.
Thirty rats were divided into three groups equally and blood samples were taken from all rats for preoperative hemoglobin (Hb) measurements. Then, the standard liver resection model was applied to all rats. Sponge for the first rat group, Ankaferd Blood Stopper® Trend-Tech for the second rat group and IvCbp plant extract mixture for the third group were applied to resection areas for 3 minutes. Liver samples of all rats were evaluated in terms of inflammation and necrosis intensity on the 5th post-operative day.
Post-operative Hb values were found as 11.0±1.1 g/dL in the sponge group, 11.9±2.0 g/dL in the Ankaferd group, and 14.1±1.2 g/dL in the IvCbp herb mixture group (p<0.001). In the histopathological examination, less necrosis was observed in the herb mixture group compared to the sponge and Ankaferd groups (p=0.001). In addition, no statistically significant necrosis difference was observed between sponge and Ankaferd groups. While less inflammation was observed in the herb mixture group compared to the other groups, Ankaferd group had the highest inflammation score (p<0.001).
IvCbp herb mixture extract group provide effective hemostatic control, caused less Hb decrease and resulted in less inflammation and necrosis compared to Ankaferd and sponge groups in a rat liver resection model.
肝外科手术中，无法有效止血和输血相关问题是导致术后死亡率和发病率的最常见原因。为了控制肝外科手术中的出血，已经使用了各种方法/药物，包括局部止血剂。本研究旨在研究传统上用作防腐剂和止血剂的旋覆花和荠苨提取物混合物（IvCbp）在大鼠肝裂伤模型中的止血特性，该提取物混合物在土耳其哈塔伊省被广泛使用。
将 30 只大鼠等分为 3 组，所有大鼠均采血进行术前血红蛋白（Hb）测量。然后，所有大鼠均应用标准肝切除术模型。第 1 组大鼠应用海绵，第 2 组大鼠应用 Ankaferd Blood Stopper® Trend-Tech，第 3 组大鼠应用 IvCbp 植物提取物混合物，均在切除区域应用 3 分钟。所有大鼠的肝组织样本均在术后第 5 天评估炎症和坏死强度。
海绵组术后 Hb 值为 11.0±1.1 g/dL，Ankaferd 组为 11.9±2.0 g/dL，IvCbp 植物混合物组为 14.1±1.2 g/dL（p<0.001）。在组织病理学检查中，与海绵和 Ankaferd 组相比，植物混合物组的坏死程度较轻（p=0.001）。此外，海绵和 Ankaferd 组之间的坏死程度无统计学差异。与其他组相比，植物混合物组的炎症程度较轻，而 Ankaferd 组的炎症评分最高（p<0.001）。
与 Ankaferd 和海绵组相比，IvCbp 植物混合物提取物组在大鼠肝切除术模型中可有效控制止血，导致 Hb 下降减少，炎症和坏死程度降低。
