P.G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, 580003, India.
Department of Chemistry, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Arch Microbiol. 2022 Aug 5;204(9):540. doi: 10.1007/s00203-022-03147-7.
During the investigation, soil actinomycetes were isolated from Kathlekanu swamp forest and the crude ethyl acetate extract from the potent isolate KF15 was analysed with GC-MS and HPTLC to identify bioactive metabolites. The crude extract was examined for in-vitro antifungal activity on pathogens of chilli; MTT cytotoxicity assay was performed against HeLa cell line to determine the anticancer potential. The isolate Streptomyces sp. strain KF15 exhibited antagonistic activity against fungal pathogens by inhibiting growth and altering growth pattern with increased antimicrobial activity in dose-dependent manner. GC-MS revealed many bioactive compounds and HPTLC depicted metabolite fingerprint. The IC of 99.85 µg/ml indicated the high potential of KF15 extract to prevent proliferation of HeLa cells. Therefore, the findings of this study indicate that the crude extract from Streptomyces sp. strain KF15 contains antifungal and anticancer metabolites; further study on purification could help in controlling many fungal diseases as well as cervical cancer in humans.
在研究过程中，从 Kathlekanu 沼泽森林中分离出土壤放线菌，并对有潜力的分离株 KF15 的粗乙酸乙酯提取物进行 GC-MS 和 HPTLC 分析，以鉴定生物活性代谢物。对粗提物进行了体外抗辣椒病原菌的活性检测；通过 MTT 细胞毒性试验对 HeLa 细胞系进行了抗癌潜力的测定。分离得到的链霉菌属 KF15 菌株通过抑制真菌病原体的生长和改变生长模式，表现出拮抗活性，并且随着抗菌活性的增加，呈剂量依赖性。GC-MS 揭示了许多生物活性化合物，HPTLC 描绘了代谢物指纹图谱。IC99.85µg/ml 表明 KF15 提取物具有阻止 HeLa 细胞增殖的高潜力。因此，本研究的结果表明，链霉菌属 KF15 菌株的粗提物含有抗真菌和抗癌代谢物；进一步的纯化研究有助于控制许多真菌病以及人类的宫颈癌。
