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源于土壤放线菌HSN-01的唑菌酯的分离、表征及其抗菌和抗癌活性

Isolation, Characterization of Pyraclostrobin Derived from Soil Actinomycete sp. HSN-01 and Its Antimicrobial and Anticancer Activity.

作者信息

Math Halaswamy Hire, Nayaka Sreenivasa, Rudrappa Muthuraj, Kumar Raju Suresh, Almansour Abdulrahman I, Perumal Karthikeyan, Kantli Girish Babu

机构信息

Post Graduate Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India.

Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

出版信息

Antibiotics (Basel). 2023 Jul 20;12(7):1211. doi: 10.3390/antibiotics12071211.

DOI:10.3390/antibiotics12071211
PMID:37508307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10376734/
Abstract

The present study demonstrated the isolation, characterization, and antimicrobial and anticancer activity of active metabolite produced from mining-soil-derived actinomycetes. Among the 21 actinomycete isolates, the isolate HSN-01 exhibited significant antimicrobial activity in primary screening and was identified as sp. through 16S rRNA gene sequencing. The active metabolite was separated, purified, and confirmed through UV-Vis spectroscopy, FTIR, HR-ESI-MS, and NMR analysis and identified as pyraclostrobin. Further, the active metabolite pyraclostrobin was tested for antimicrobial and anticancer activity against the hepatocellular carcinoma (HepG2) cell line. The metabolite exhibited maximum antimicrobial potential with 17.0, 13.33, 17.66, 15.66, 14.66, and 14.0 mm of inhibition against , , , , , and . The active metabolite exhibited dose-dependent anticancer potential against the hepatocellular carcinoma (HepG2) cell line with the IC 56.76 µg/mL. This study suggests that sp. HSN-01 is an excellent source of active secondary metabolites with various biological activities.

摘要

本研究展示了从采矿土壤中分离出的放线菌产生的活性代谢产物的分离、表征及其抗菌和抗癌活性。在21株放线菌分离物中，分离株HSN-01在初步筛选中表现出显著的抗菌活性，并通过16S rRNA基因测序鉴定为 种。活性代谢产物通过紫外可见光谱、傅里叶变换红外光谱、高分辨电喷雾电离质谱和核磁共振分析进行分离、纯化和确认，并鉴定为吡唑醚菌酯。此外，还对活性代谢产物吡唑醚菌酯针对肝癌（HepG2）细胞系进行了抗菌和抗癌活性测试。该代谢产物对 、 、 、 、 和 的抑菌圈直径分别为17.0、13.33、17.66、15.66、14.66和14.0毫米，展现出最大抗菌潜力。活性代谢产物对肝癌（HepG2）细胞系表现出剂量依赖性抗癌潜力，IC 为56.76 µg/mL。本研究表明， 种HSN-01是具有多种生物活性的活性次生代谢产物的优良来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/a11441d26a2d/antibiotics-12-01211-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/ef2db5601797/antibiotics-12-01211-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/c0f98d65c054/antibiotics-12-01211-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/b17f19cb3e9e/antibiotics-12-01211-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/f4e2532af51a/antibiotics-12-01211-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/859a995387e1/antibiotics-12-01211-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/7c2afccf0623/antibiotics-12-01211-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/d31e18609f79/antibiotics-12-01211-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/e5d3c5833370/antibiotics-12-01211-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/4daa40966642/antibiotics-12-01211-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/8aacac34b672/antibiotics-12-01211-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/a11441d26a2d/antibiotics-12-01211-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/ef2db5601797/antibiotics-12-01211-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/c0f98d65c054/antibiotics-12-01211-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/b17f19cb3e9e/antibiotics-12-01211-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/f4e2532af51a/antibiotics-12-01211-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/859a995387e1/antibiotics-12-01211-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/7c2afccf0623/antibiotics-12-01211-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/d31e18609f79/antibiotics-12-01211-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/e5d3c5833370/antibiotics-12-01211-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/4daa40966642/antibiotics-12-01211-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/8aacac34b672/antibiotics-12-01211-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/676f/10376734/a11441d26a2d/antibiotics-12-01211-g011.jpg

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