School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, China.
Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
Biomater Adv. 2022 May;136:212795. doi: 10.1016/j.bioadv.2022.212795. Epub 2022 Apr 10.
Patients with acute and chronic wounds have been increasing around the world, and the demand for wound treatment and care is also increasing. Therefore, a new nanofiber wound dressing should be prepared to promote the wound healing process. In this study, we report the design and preparation of a hierarchical structural film wound dressing. The top layer is composed of profoundly hydrophobic polycaprolactone (PCL), which is used to resist the adhesion of external microorganisms. The bottom layer is made of hydrophilic gelatin, which provides a moist healing environment for the wound. The middle layer is composed of hydrophilic Janus nanofibers prepared with the latest side-by-side electrospinning technique. Gelatin and PCL are used as polymer matrices loaded with the ciprofloxacin (CIP) drug and zinc oxide nanoparticles (n-ZnO), respectively. Test results show that the dressing has outstanding surface wettability, excellent mechanical properties, and rapid drug release. The presence of biologically active ingredients provides antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Finally, the results of wound healing in mice show accelerated collagen deposition, promotion of angiogenesis, and complete wound healing within 14 days. Overall, this hierarchical structural dressing has a strong potential for accelerating wound healing.
全球范围内急性和慢性伤口患者不断增加，对伤口治疗和护理的需求也在不断增加。因此，需要制备新型纳米纤维伤口敷料以促进伤口愈合过程。在本研究中，我们报告了分层结构薄膜伤口敷料的设计和制备。顶层由深度疏水性聚己内酯（PCL）组成，用于抵抗外部微生物的黏附。底层由亲水性明胶组成，为伤口提供湿润的愈合环境。中间层由最新的并列静电纺丝技术制备的亲水性 Janus 纳米纤维组成。明胶和 PCL 分别用作载有环丙沙星（CIP）药物和氧化锌纳米颗粒（n-ZnO）的聚合物基质。测试结果表明，该敷料具有出色的表面润湿性、优异的机械性能和快速的药物释放。生物活性成分的存在提供了针对金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）的抗菌活性。最后，小鼠伤口愈合结果表明，该敷料能加速胶原蛋白沉积、促进血管生成，并在 14 天内完全愈合伤口。总的来说，这种分层结构敷料具有加速伤口愈合的巨大潜力。
