Laboratory of Neuroproteomics, Department of Cell and Tissue Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil.
Adv Exp Med Biol. 2022;1400:129-138. doi: 10.1007/978-3-030-97182-3_10.
The mass spectrometer is an instrument that observes particular masses of molecules of interest. Over the past century, it has grown to become a highly sensitive and robust tool in laboratorial and clinical research to identify and quantify thousands of proteins in a given sample in an unbiased manner leading to the quick rise in its use. This unbiased and high-throughput nature is extremely important in discovery-based studies, since no preset targets can be selected, as is the case with several other proteomic methods. In studying multifactorial diseases such as schizophrenia, mass-spectrometry-based proteomics has been frequently used and new improvements to the technique have been quickly taken advantage of. Over the past 15 years, mass spectrometry has evolved greatly, and with it, the proteomic analyses and data have evolved. In this chapter, a brief history of the evolution of mass spectrometry is covered along with how schizophrenia research has grown alongside this valuable methodology.
质谱仪是一种观察特定分子质量的仪器。在过去的一个世纪里，它已经发展成为实验室和临床研究中一种高度敏感和强大的工具，可以以非偏见的方式识别和定量给定样本中的数千种蛋白质，这导致了它的快速应用。这种无偏见和高通量的性质在基于发现的研究中非常重要，因为与其他几种蛋白质组学方法不同，不能选择预设的目标。在研究精神分裂症等多因素疾病时，基于质谱的蛋白质组学经常被使用，并且该技术的新改进也被迅速利用。在过去的 15 年中，质谱技术有了很大的发展，与之相关的蛋白质组学分析和数据也得到了发展。在本章中，涵盖了质谱技术的发展简史，以及精神分裂症研究是如何随着这种有价值的方法而发展的。
