Schofield P S, French T J, Sugden M C
Biochem J. 1987 Jan 15;241(2):475-81. doi: 10.1042/bj2410475.
Rats were subjected to laparotomy, or laparotomy and partial hepatectomy, at 0-48 h before administration of water or medium-chain-length triacylglycerol, having been starved post-operatively. Functional hepatectomies were performed at intervals after the intragastric load. Blood ketone-body concentrations after medium-chain triacylglycerol administration and/or functional hepatectomy of these rats were compared with values obtained in starved control rats. Decreased ketonaemia in response to medium-chain triacylglycerol was observed for up to 48 h after partial hepatectomy and at 1 and 2 h after laparotomy, but not at 24 or 48 h after laparotomy. Rates of ketone-body clearance after functional hepatectomy were unaffected by prior laparotomy or partial hepatectomy. Ketonaemia after medium-chain-triacylglycerol administration was only partially blocked by inhibition of CPT I (carnitine palmitoyltransferase I). The results demonstrate sustained effects of partial hepatectomy and short-term effects of surgical stress to decrease ketonaemia via inhibition of ketogenesis at site(s) distal to CPT I.
在给予水或中链长度三酰甘油前0 - 48小时，对大鼠进行剖腹手术，或剖腹手术加部分肝切除术，术后使其饥饿。在胃内负荷后每隔一段时间进行功能性肝切除术。将这些大鼠给予中链三酰甘油和/或进行功能性肝切除术后的血酮体浓度与饥饿对照大鼠的值进行比较。部分肝切除术后长达48小时以及剖腹手术后1小时和2小时观察到中链三酰甘油引起的酮血症降低，但剖腹手术后24小时或48小时未观察到。功能性肝切除术后酮体清除率不受先前剖腹手术或部分肝切除术的影响。中链三酰甘油给药后的酮血症仅部分被肉碱棕榈酰转移酶I（CPT I）抑制所阻断。结果表明，部分肝切除术具有持续作用，手术应激具有短期作用，可通过抑制CPT I远端部位的酮体生成来降低酮血症。
