Bhattacharjee Surjit, Paul Abhirup, Jana Aradhana, Unnati G Meher, R Deepak, Miao Ye, Lu Honglin, Shen Guoxin, Mishra Neelam
Bengaluru, Karnataka, India.
Department of Botany, St. Joseph's University, Bengaluru, Karnataka, India.
PeerJ. 2025 May 27;13:e19431. doi: 10.7717/peerj.19431. eCollection 2025.
Protein phosphatases (PP) play a crucial role in regulation of physiological response to various environmental stimuli in plants. Protein phosphatase 2A (PP2A) are a class of Ser/Thr protein phosphatases which are present widely across plant species and facilitate plant responses to biotic and abiotic stresses, and hormone treatment.
Using BLASTp, tea PP2A genes were found and their physicochemical characteristics (ExPASy), subcellular localization, and intron-exon structure (GSDS) were described. MEGA software was used to examine phylogenetic relationships, SMART online tool to analyze conserved domains, and PlantCARE tool to analyze cis-elements. The Heatmapper online tool was used to visualize expression profiles across plant tissues and stress conditions. Under controlled conditions, 2-year-old plants were exposed to methyl jasmonate (MeJA) stress treatments, cold drought, and salt stresses and qRT-PCR (2 technique, CsACTIN as internal control) was used to validate the results.
We found 11 PP2A genes in () through a genome-wide search wherein was taken as the reference genome. Further, a systematic study was conducted wherein physicochemical properties, phylogeny, gene structure and gene expression were explored. Analysis of cis-elements, gene ontology, and expression patterns of genes under various stresses, combined with prior research on the PP2A gene family in other plants, suggests that the PP2A family plays a role in regulating phytohormone (auxin, salicylic acid, gibberellin, abscisic acid, MeJA) responses during stress in . The real time PCR also confirmed the potential role of (TEA018948.1) gene in abiotic stress responses.
This study offers potential goals for further in-depth investigation and functional analysis, alongside enhancing our understanding of the regulatory network of PP2A genes in .
蛋白磷酸酶（PP）在植物对各种环境刺激的生理反应调节中起关键作用。蛋白磷酸酶2A（PP2A）是一类丝氨酸/苏氨酸蛋白磷酸酶，广泛存在于植物物种中，促进植物对生物和非生物胁迫以及激素处理的反应。
使用BLASTp搜索茶树PP2A基因，并描述其理化特性（ExPASy）、亚细胞定位和内含子-外显子结构（GSDS）。使用MEGA软件检查系统发育关系，利用SMART在线工具分析保守结构域，并用PlantCARE工具分析顺式元件。使用Heatmapper在线工具可视化植物组织和胁迫条件下的表达谱。在可控条件下，对2年生植株进行茉莉酸甲酯（MeJA）胁迫处理、冷胁迫、干旱胁迫和盐胁迫，并使用qRT-PCR（以CsACTIN作为内参基因的技术）验证结果。
以（）为参考基因组，通过全基因组搜索在（）中发现了11个PP2A基因。此外，还进行了系统研究，探索了其理化性质、系统发育、基因结构和基因表达。对顺式元件、基因本体以及各种胁迫下基因表达模式的分析，结合之前对其他植物中PP2A基因家族的研究，表明PP2A家族在（）胁迫期间调节植物激素（生长素、水杨酸、赤霉素、脱落酸、MeJA）反应中发挥作用。实时PCR也证实了（TEA018948.1）基因在非生物胁迫反应中的潜在作用。
本研究为进一步深入研究和功能分析提供了潜在目标，同时增进了我们对（）中PP2A基因调控网络的理解。
