PG & Research Department of Biochemistry, D.K.M College (Autonomous),Vellore 632001, Tamil Nadu, India.
PG & Research Department of Chemistry, C. Abdul Hakeem College (Autonomous), Melvisharam 632509, Tamil Nadu, India.
Int J Biol Macromol. 2022 Oct 31;219:304-311. doi: 10.1016/j.ijbiomac.2022.08.007. Epub 2022 Aug 4.
The present work aims at evaluating the in vitro biocompatibility, antibacterial activity and antioxidant capacity of the fabricated and optimized Alginate/Chitosan nanoparticles (ALG/CSNPs) and quercetin loaded Alginate/Chitosan nanoparticles (Q-ALG/CSNPs) with an improved biological efficacy on the hydrophobic flavonoid.The physicochemical properties were determined by TEM and FTIR analysis. The nanoparticles evaluated for the encapsulation of quercetin exerted % encapsulation efficiency (EE) that varied between 76 and 82.4 % and loading capacity (LC) from 31 to 46.5 %. Potential cytotoxicity of the ALG/CSNPs and Q-ALG/CSNPs upon L929 fibroblast cell line was evaluated by MTT reduction Assay and expressed as % cell viability. The in vitro antibacterial property was studied by well diffusion method against gram-positive bacteria Staphylococcus aureus (ATCC 25925) and gram-negative bacteria Escherichia coli (ATCC 25923). The inhibitory efficacy by scavenging free radical intermediates was evaluated by 1,1, diphenyl 2-picrylhydrazyl (DPPH) assay. The results of in vitro cytotoxicity showed biocompatibility towards L929 cells. Quercetin loaded Alginate/Chitosan nanoparticles inhibited the growth of microorganisms than pure quercetin. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging results have shown a high level of antioxidant property for encapsulated Quercetin in Alginate/Chitosan nanoparticles compared to free Quercetin. The findings of our study suggest that the developed ALG/CSNPs and Q-ALG/CSNPs possess the prerequisites and be proposed as a suitable system for delivering quercetin with enhanced therapeutic effectuality.
本工作旨在评估制备和优化的海藻酸钠/壳聚糖纳米粒子（ALG/CSNPs）和负载槲皮素的海藻酸钠/壳聚糖纳米粒子（Q-ALG/CSNPs）的体外生物相容性、抗菌活性和抗氧化能力，以提高疏水性黄酮类化合物的生物功效。通过 TEM 和 FTIR 分析确定了纳米粒子的物理化学性质。评估了用于包封槲皮素的纳米粒子的包封效率（EE）在 76%至 82.4%之间，载药量（LC）在 31%至 46.5%之间。通过 MTT 还原测定法评估了 ALG/CSNPs 和 Q-ALG/CSNPs 对 L929 成纤维细胞系的潜在细胞毒性，并表示为细胞活力的百分比。通过平板扩散法研究了体外抗菌性能，对抗革兰氏阳性菌金黄色葡萄球菌（ATCC 25925）和革兰氏阴性菌大肠杆菌（ATCC 25923）。通过 1,1-二苯基-2-苦基肼基（DPPH）测定法评估了清除自由基中间体的抑制效果。体外细胞毒性结果表明对 L929 细胞具有生物相容性。负载槲皮素的海藻酸钠/壳聚糖纳米粒子抑制微生物生长的能力优于纯槲皮素。1,1-二苯基-2-苦基肼基（DPPH）自由基清除结果表明，与游离槲皮素相比，包封在海藻酸钠/壳聚糖纳米粒子中的槲皮素具有更高的抗氧化性能。我们的研究结果表明，开发的 ALG/CSNPs 和 Q-ALG/CSNPs 具有必要的先决条件，并被提议作为一种具有增强治疗效果的递送槲皮素的合适系统。
