State Key Laboratory of Analytical Chemistry for Life Science, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Collaborative Innovation Center of Advanced Microstructures, Chemistry and Biomedicine Innovation Center, Nanjing, 210023, China.
Shenzhen Research Institute of Nanjing University, Shenzhen, 518000, China.
Angew Chem Int Ed Engl. 2022 Oct 4;61(40):e202208290. doi: 10.1002/anie.202208290. Epub 2022 Aug 25.
DNA nanotechnology has provided credible approaches for assembly of three-dimensional (3D) lattices with complex patterns. However, the symmetries are strictly dependent on their initial configurations and difficult to alter via non-thermal treatments. While switchable nucleic acid structures have been employed to construct deformable DNA motifs, it remains challenging to arrange them anisotropically in 3D lattices to trigger directed collective shape transition and dynamic symmetry conversion. In this work, we used octahedral DNA origami frames to synthesize four DNA origami lattices by placing the pH-reactive i-motif sequences in the desired dimensions. Thereinto, lattices with an anisotropic design can switch between simple cubic (SC) and simple tetragonal (ST) upon pH change. Small angle X-ray scattering (SAXS) results reveal the feasibility of obtaining 3D lattices with sensitive responses to external stimuli, expanding the way to obtain low-symmetry lattices.
DNA 纳米技术为具有复杂图案的三维（3D）晶格的组装提供了可靠的方法。然而，其对称性严格依赖于初始构型，并且难以通过非热处理进行改变。虽然可切换核酸结构已被用于构建可变形 DNA 基元，但在 3D 晶格中各向异性地排列它们以触发定向集体形状转变和动态对称转换仍然具有挑战性。在这项工作中，我们使用八面体 DNA 折纸框架，通过在所需尺寸处放置 pH 反应性 i-motif 序列来合成四个 DNA 折纸晶格。其中，具有各向异性设计的晶格可以在 pH 变化时在简单立方（SC）和简单四方（ST）之间切换。小角 X 射线散射（SAXS）结果表明获得对外界刺激具有敏感响应的 3D 晶格的可行性，扩展了获得低对称晶格的方法。
