超声联合多孔脂质氯碘羟喹/聚乳酸-羟基乙酸共聚物微泡对异丙肾上腺素攻击诱导的HL-1心肌细胞铁死亡的治疗作用
Therapeutic Effect of Ultrasound Combined With Porous Lipid Clioquinol/PLGA Microbubbles on Ferroptosis in HL-1 Cardiac Cell Induced by Isoproterenol Attack.
作者信息
Li Nana, Dong Lei, Shen Yuanyuan, Wang Yongling, Chang Liansheng, Wu Hongwei, Chang Yuqiao, Li Menghao, Li Dan, Li Zhaoyi, He Mei, Li Cheng, Wei Yao, Xie Haiqin, Wang Feng
机构信息
Henan Key Laboratory of Medical Tissue Regeneration, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.
National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China.
出版信息
Front Pharmacol. 2022 Jul 22;13:918292. doi: 10.3389/fphar.2022.918292. eCollection 2022.
In recent years, studies have shown a close relationship between cardiomyocyte death and ferroptosis. Clioquinol (CQ) can inhibit ferroptosis. Porous lipid-poly (lactic-co-glycolic acid) (PLGA) microbubbles (MBs) were prepared by double emulsification (W/O/W) using 1,2-dioctadecanoyl-sn-glycero-3-phophocholine and PLGA as raw materials. Porous lipid-PLGA MBs were used as carriers to prepare CQ/PLGA MBs containing CQ. CQ/PLGA had the advantages of high drug loading, good biocompatibility, and sustained release. Our results showed that CQ/PLGA improved the effect of CQ and reduced its cytotoxicity. Under low-frequency ultrasound with certain parameters, CQ/PLGA showed steady-state cavitation, which increased the membrane permeability of mouse cardiomyocyte HL-1 to a certain extent and further prevented the process of ferroptosis in mouse cardiomyocyte HL-1.
近年来，研究表明心肌细胞死亡与铁死亡之间存在密切关系。氯碘羟喹（CQ）可抑制铁死亡。以1,2-二油酰基-sn-甘油-3-磷酸胆碱和聚乳酸-羟基乙酸共聚物（PLGA）为原料，通过复乳化法（W/O/W）制备了多孔脂质-聚乳酸-羟基乙酸共聚物（PLGA）微泡（MBs）。以多孔脂质-PLGA微泡为载体，制备了载有CQ的CQ/PLGA微泡。CQ/PLGA具有载药量高、生物相容性好和缓释的优点。我们的结果表明，CQ/PLGA提高了CQ的效果并降低了其细胞毒性。在具有特定参数的低频超声作用下，CQ/PLGA表现出稳态空化，在一定程度上增加了小鼠心肌细胞HL-1的膜通透性，并进一步阻止了小鼠心肌细胞HL-1的铁死亡过程。
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