Wayner D D, Burton G W, Ingold K U, Barclay L R, Locke S J
Biochim Biophys Acta. 1987 Jun 22;924(3):408-19. doi: 10.1016/0304-4165(87)90155-3.
The Total (Peroxyl) Radical-trapping Antioxidant Parameter (TRAP) of six freshly prepared human plasma samples and 45 frozen plasma samples has been determined. It is shown that contributions from urate (35-65%), plasma proteins (10-50%), ascorbate (0-24%) and vitamin E (5-10%) to TRAP account for all of the peroxyl radical-trapping antioxidant activity in the majority of the samples. The changes in concentrations of the plasma antioxidants during peroxyl radical attack show that the first line of defense is provided by the plasma sulfhydryl groups, even urate being spared during the initial stages of the reaction. The modes of action of all of these plasma antioxidants and possible interactions between them are discussed, with particular emphasis on the abilities of the water-soluble antioxidants to regenerate or spare the only lipid-soluble antioxidant, vitamin E.
已测定6份新鲜制备的人血浆样本和45份冷冻血浆样本的总（过氧自由基）自由基捕获抗氧化参数（TRAP）。结果表明，在大多数样本中，尿酸盐（35 - 65%）、血浆蛋白（10 - 50%）、抗坏血酸盐（0 - 24%）和维生素E（5 - 10%）对TRAP的贡献占所有过氧自由基捕获抗氧化活性。过氧自由基攻击期间血浆抗氧化剂浓度的变化表明，第一道防线由血浆巯基提供，甚至在反应初始阶段尿酸盐也未受到影响。讨论了所有这些血浆抗氧化剂的作用方式以及它们之间可能的相互作用，特别强调了水溶性抗氧化剂再生或节省唯一脂溶性抗氧化剂维生素E的能力。
