Wayner D D, Burton G W, Ingold K U, Locke S
FEBS Lett. 1985 Jul 22;187(1):33-7. doi: 10.1016/0014-5793(85)81208-4.
Plasma obtained from fasted humans has been analysed for total radical-trapping antioxidant content by subjecting it to controlled peroxidation using the thermal decomposition of water-soluble azobis (2-amidinopropane hydrochloride) at 37 degrees C to produce peroxyl radicals at a known, steady rate. It is found that the total radical-trapping antioxidant content is rather similar for the 7 subjects that have been tested and, furthermore, it is 10-20-times larger than the effect attributable to vitamin E alone. Although it is shown that urate and ascorbate augment the contribution from vitamin E, their contributions (21-34 and 0-2%, respectively) still leave 57-73% of the total antioxidant content unaccounted for. Evidence is presented to show that this previously unrecognized large reserve of antioxidant capacity is attributable to the plasma proteins.
通过在37摄氏度下利用水溶性偶氮二（2-脒基丙烷盐酸盐）的热分解以已知的稳定速率产生过氧自由基，对禁食人类的血浆进行受控过氧化，分析了血浆中的总自由基捕获抗氧化剂含量。结果发现，所测试的7名受试者的总自由基捕获抗氧化剂含量相当相似，此外，其含量比仅归因于维生素E的效果大10至20倍。虽然已表明尿酸盐和抗坏血酸盐增强了维生素E的贡献，但它们的贡献（分别为21 - 34%和0 - 2%）仍使总抗氧化剂含量的57 - 73%无法解释。有证据表明，这种先前未被认识到的大量抗氧化能力储备归因于血浆蛋白。
