Pincemail Joël, Rousseau Anne-Françoise, Kaux Jean-François, Cheramy-Bien Jean-Paul, Bruyère Christine, Prick Jeanine, Stern David, Kaci Mouna-Messaouda, Maertens De Noordhout Benoît, Albert Adelin, Eubelen Céline, Goff Caroline Le, Misset Benoît, Cavalier Etienne, Charlier Corinne, Meziane Smail
Clinical Chemistry, University Hospital of Liège, Sart Tilman, 4000 Liège, Belgium.
Intensive Care Department, University Hospital of Liège, Sart Tilman, 4000 Liège, Belgium.
Biomedicines. 2023 Apr 28;11(5):1308. doi: 10.3390/biomedicines11051308.
Oxidative stress (OS) could cause various COVID-19 complications. Recently, we have developed the Pouvoir AntiOxydant Total (PAOT®) technology for reflecting the total antioxidant capacity (TAC) of biological samples. We aimed to investigate systemic oxidative stress status (OSS) and to evaluate the utility of PAOT® for assessing TAC during the recovery phase in critical COVID-19 patients in a rehabilitation facility.
In a total of 12 critical COVID-19 patients in rehabilitation, 19 plasma OSS biomarkers were measured: antioxidants, TAC, trace elements, oxidative damage to lipids, and inflammatory biomarkers. TAC level was measured in plasma, saliva, skin, and urine, using PAOT and expressed as PAOT-Plasma, -Saliva, -Skin, and -Urine scores, respectively. Plasma OSS biomarker levels were compared with levels from previous studies on hospitalized COVID-19 patients and with the reference population. Correlations between four PAOT scores and plasma OSS biomarker levels were analyzed.
During the recovery phase, plasma levels in antioxidants (γ-tocopherol, β-carotene, total glutathione, vitamin C and thiol proteins) were significantly lower than reference intervals, whereas total hydroperoxides and myeloperoxidase (a marker of inflammation) were significantly higher. Copper negatively correlated with total hydroperoxides (r = 0.95, = 0.001). A similar, deeply modified OSS was already observed in COVID-19 patients hospitalized in an intensive care unit. TAC evaluated in saliva, urine, and skin correlated negatively with copper and with plasma total hydroperoxides. To conclude, the systemic OSS, determined using a large number of biomarkers, was always significantly increased in cured COVID-19 patients during their recovery phase. The less costly evaluation of TAC using an electrochemical method could potentially represent a good alternative to the individual analysis of biomarkers linked to pro-oxidants.
氧化应激（OS）可导致多种新冠病毒疾病（COVID-19）并发症。最近，我们开发了总抗氧化能力（PAOT®）技术来反映生物样本的总抗氧化能力（TAC）。我们旨在调查康复机构中重症COVID-19患者恢复阶段的全身氧化应激状态（OSS），并评估PAOT®在评估TAC方面的效用。
共对12名正在康复的重症COVID-19患者进行了检测，测定了19种血浆OSS生物标志物：抗氧化剂、TAC、微量元素、脂质氧化损伤和炎症生物标志物。使用PAOT分别在血浆、唾液、皮肤和尿液中测量TAC水平，并分别表示为PAOT-血浆、-唾液、-皮肤和-尿液评分。将血浆OSS生物标志物水平与先前关于住院COVID-19患者的研究水平以及参考人群水平进行比较。分析了四个PAOT评分与血浆OSS生物标志物水平之间的相关性。
在恢复阶段，抗氧化剂（γ-生育酚、β-胡萝卜素、总谷胱甘肽、维生素C和硫醇蛋白）的血浆水平显著低于参考区间，而总氢过氧化物和髓过氧化物酶（一种炎症标志物）则显著升高。铜与总氢过氧化物呈负相关（r = 0.95，P = 0.001）。在重症监护病房住院的COVID-19患者中也观察到了类似的、深度改变的OSS。在唾液、尿液和皮肤中评估的TAC与铜和血浆总氢过氧化物呈负相关。总之，使用大量生物标志物确定的全身OSS在康复的COVID-19患者恢复阶段始终显著升高。使用电化学方法对TAC进行成本较低的评估可能是与促氧化剂相关生物标志物个体分析的一个很好的替代方法。
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