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A、B、C、G和L组链球菌脱氧核糖核酸酶的证实与特性研究

The demonstration and characterization of deoxyribonucleases of streptococci group A, B, C, G and L.

作者信息

Gudding R

出版信息

Acta Vet Scand. 1979;20(1):102-21. doi: 10.1186/BF03546634.

DOI:10.1186/BF03546634

PMID:35939

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8322875/

Abstract

Streptococcus pyogenes, S. agalactiae, S. dysgalactiae, S. equi, S. equisimilis, S. zooepidemicus, Streptococcus group G and L were found to produce deoxyribonucleases (DNases) which were demonstrated using the Toluidine Blue DNA Agar (TDA) described for staphylococcal DNases. The activity of streptococcal DNases increased in the presence of Mg and Ca ions, the pH optimum was about 7.5 and native DNA was the best enzyme substrate. It is consequently recommended to modify the TDA according to these results for the demonstration of streptococcal DNases. All streptococcal DNases, except the DNase of S. zooepidemicus, were found to be heat-stable. Isoelectric focusing was a convenient technique for separation of streptococcal DNases and for estimation of the pI values of the DNases. S. agalactiae and S. dysgalactiae generally exhibited distinct species specific patterns in the isoelectric focusing experiments. The DNases produced by S. pyogenes were serologically related to the DNases of S. dysgalactiae and Streptococcus group G. A similar relationship was demonstrated between the DNases produced by S. equisimilis and Streptococcus group L.

摘要

已发现化脓性链球菌、无乳链球菌、停乳链球菌、马链球菌、类马链球菌、兽疫链球菌、G群和L群链球菌可产生脱氧核糖核酸酶（DNases），使用针对葡萄球菌DNases描述的甲苯胺蓝DNA琼脂（TDA）可证实这一点。链球菌DNases的活性在镁离子和钙离子存在时增强，最适pH约为7.5，天然DNA是最佳酶底物。因此，建议根据这些结果对TDA进行修改，以用于链球菌DNases的鉴定。除兽疫链球菌的DNase外，所有链球菌DNases均具有热稳定性。等电聚焦是分离链球菌DNases和估计DNases的等电点（pI）值的便捷技术。在等电聚焦实验中，无乳链球菌和停乳链球菌通常呈现出明显的种特异性模式。化脓性链球菌产生的DNases与停乳链球菌和G群链球菌的DNases在血清学上相关。类马链球菌和L群链球菌产生的DNases之间也显示出类似关系。