Physiological, biochemical and molecular evaluation of mungbean genotypes for agronomical yield under drought and salinity stresses in the presence of humic acid.

Drought and salinity are potential threats in arid and semi arid regions. The current study was conducted with objective to optimize the production of different exotic genotypes of mungbean (NM-121-25, Chakwal M-6, DM-3 and PRI-Mung-2018) under drought and salinity stresses using humic acid in field experiments. One year tri-replicate field experiment was performed in RCBD using three factorial arrangement and effects of humic acid (60 kg ha) were evaluated at physiological, biochemical, molecular and agronomical level under individual and integrated applications of drought (no irrigation till 15 days) and salinity (EC 6.4 dSM). Data for physiological parameters (total chlorophyll, photosynthesis rate, stomatal conductance, transpiration rate and membrane damage), antioxidant enzymes (superoxide dismutase, catalase, peroxidase) and proline were collected on weekly basis since after the initiation of drought and salinity stresses. However data for agronomic characteristics (plant height, branches plant, LAI, pods plant, pod length and hundred seed weight) and grain carbohydrate content were collected after harvesting, while sampling for drought () and salinity () related genes expression study was done after plants attained seedling stage. Under both individual and integrated applications of drought and salinity, all genotypes showed significant ( ≤ 0.05) increase in all traits excluding Cell membrane damage and proline during humic acid application. Likewise, genes expression revealed statistically distinct ( ≤ 0.05) up-regulation under humic acid treatment as compared to no humic acid treatment during both individual and integrated applications of drought and salinity. The genotype PRI-Mung-2018 recorded noteworthy performance during study. Moreover correlation and PCA analysis revealed that ultimate agronomical yield due to humic acid is an outcome of interconnection of physiological and biochemical parameters.