School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Shenzhen Institute of Research and Innovation, University of Hong Kong, Shenzhen, China.
Phytother Res. 2022 Aug;36(8):3232-3247. doi: 10.1002/ptr.7452.
The current COVID-19 pandemic caused by SARS-Cov-2 is responsible for more than 6 million deaths globally. The development of broad-spectrum and cost-effective antivirals is urgently needed. Medicinal plants are renowned as a complementary approach in which antiviral natural products have been established as safe and effective drugs. Here, we report that the percolation extract of Spatholobus suberectus Dunn (SSP) is a broad-spectrum viral entry inhibitor against SARS-CoV-1/2 and other enveloped viruses. The viral inhibitory activities of the SSP were evaluated by using pseudotyped SARS-CoV-1 and 2, HIV-1 , and H5N1. SSP effectively inhibited viral entry and with EC values ranging from 3.6 to 5.1 μg/ml. Pre-treatment of pseudovirus or target cells with SSP showed consistent inhibitory activities with the respective EC value of 2.3 or 2.1 μg/ml. SSP blocked both SARS-CoV-2 spike glycoprotein and the host ACE2 receptor. In vivo studies indicated that there was no abnormal toxicity and behavior in long-term SSP treatment. Based on these findings, we concluded that SSP has the potential to be developed as a drug candidate for preventing and treating COVID-19 and other emerging enveloped viruses.
由 SARS-CoV-2 引起的当前 COVID-19 大流行在全球范围内造成超过 600 万人死亡。急需开发广谱且具有成本效益的抗病毒药物。药用植物是一种著名的辅助治疗方法，其中抗病毒天然产物已被确立为安全有效的药物。在这里，我们报告称鸡血藤（SSP）的渗滤提取物是一种广谱的病毒进入抑制剂，可对抗 SARS-CoV-1/2 和其他包膜病毒。通过使用假型 SARS-CoV-1 和 2、HIV-1 和 H5N1 评估了 SSP 的病毒抑制活性。SSP 有效抑制病毒进入，EC 值范围为 3.6 至 5.1μg/ml。用 SSP 预处理假病毒或靶细胞显示出一致的抑制活性，相应的 EC 值分别为 2.3 或 2.1μg/ml。SSP 可阻断 SARS-CoV-2 刺突糖蛋白和宿主 ACE2 受体。体内研究表明，长期 SSP 治疗没有异常的毒性和行为。基于这些发现，我们得出结论，SSP 有可能被开发为预防和治疗 COVID-19 和其他新兴包膜病毒的候选药物。
