Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, CEP 14040-901 Ribeirão Preto-SP, Brazil.
Departamento de Engenharia de Alimentos, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Av. Duque de Caxias Norte 225, CEP 13635-900 Pirassununga-SP, Brazil.
Food Chem. 2022 Dec 15;397:133857. doi: 10.1016/j.foodchem.2022.133857. Epub 2022 Aug 4.
The objectives of this study were to extract bioactive compounds from carrot by-products and evaluate their chemical stability after encapsulation in liposomes (L) coated either with chitosan (Ch) or using sodium tripolyphosphate for chitosan complexation (TPP-Ch). The main compounds quantified in this study were carotenoids and total phenolic compounds, which reached encapsulation efficiencies higher than 75%. The TPP-Ch charged with carrot extract showed greater particle size (90.5 nm) and zeta potential (+22 mV) than vesicles without coating (68.0 nm and -2 mV, respectively), indicating that liposomes were successfully coated with chitosan. Regarding results of the carotenoid's encapsulated stability, TPP-Ch particles were more efficient preventing their degradation in all the experimental conditions studied (40 and 70 °C). It is significant that loaded TPP-Ch particles demonstrated similar results for the stability of carotenoid-rich extracts in ethanol, which would therefore be suitable for application in food industry or any aqueous matrices.
本研究的目的是从胡萝卜副产物中提取生物活性化合物，并评估其在包封于脂质体（L）后的化学稳定性，脂质体的外壳分别用壳聚糖（Ch）或三聚磷酸钠（TPP）进行壳聚糖复合（TPP-Ch）。本研究中定量的主要化合物为类胡萝卜素和总酚类化合物，其包封效率均高于 75%。用胡萝卜提取物荷正电的 TPP-Ch 的粒径（90.5nm）和zeta 电位（+22mV）大于未涂层的囊泡（分别为 68.0nm 和-2mV），表明脂质体成功地用壳聚糖进行了涂层。关于类胡萝卜素包封稳定性的结果，在所有研究的实验条件下（40 和 70°C），TPP-Ch 颗粒更有效地防止了它们的降解。重要的是，负载 TPP-Ch 颗粒在乙醇中对富含类胡萝卜素的提取物的稳定性表现出相似的结果，因此适用于食品工业或任何水基基质的应用。
