Department of Animal, Plant and Soil Sciences, School of Agriculture, Biomedicine and Environment, La Trobe University, Bundoora, Victoria, Australia.
PeerJ. 2022 Aug 4;10:e13778. doi: 10.7717/peerj.13778. eCollection 2022.
, commonly referred to as liver flukes, is a substantial zoonotic parasitic disease of humans and livestock globally. While infection is readily controlled by anthelmintics, namely triclabendazole, the heavy reliance on triclabendazole has resulted in drug resistance appearing worldwide. Due to drug resistance, it is imperative to adopt an integrated parasite management program to preserve the efficacy of currently available anthelmintics. A integrated liver fluke management plan would benefit from a simple rapid, field-deployable diagnostic for detection of in environment and the host. Therefore, a rapid DNA test using loop-mediated isothermal amplification was developed and optimised for the detection of from faecal and water samples to enable the detection of parasites both within the host and from the environment. The assay presented here is fast, with amplification in ≤20 min, and highly sensitive, with a detection limit of 5 × 10 ng/µL. The workflow presented here provides a time to result of ≤60 min without requiring a commercial kit for the extraction of DNA from faecal and water samples, and pending further validation from field-samples, could potentially be used to enable real-time decision making to mitigate parasite prevalence on a farming property and with no requirement for sample transportation.
肝片形吸虫，通常被称为肝吸虫，是一种在全球范围内对人类和家畜具有重要意义的人畜共患寄生虫病。虽然驱虫药（如三氯苯达唑）可有效控制感染，但由于对三氯苯达唑的严重依赖，该药物在全球范围内已出现耐药性。由于耐药性的出现，必须采取综合寄生虫管理计划来保持现有驱虫药的疗效。一个综合的肝吸虫管理计划将受益于一种简单、快速、可在现场部署的诊断方法，用于检测环境和宿主中的 。因此，开发并优化了一种使用环介导等温扩增的快速 DNA 检测方法，用于从粪便和水样中检测 ，从而能够在宿主内和从环境中检测寄生虫。该检测方法快速，扩增时间在 20 分钟以内，灵敏度高，检测限为 5×10ng/µL。本文提出的工作流程在无需使用商用试剂盒从粪便和水样中提取 DNA 的情况下，可在 60 分钟内得出结果，并且在等待进一步验证来自现场样本的结果后，该方法可能可用于实时决策，以减轻农场寄生虫病的流行，并无需进行样本运输。
