Wang Dan, Yang Yani, Chen Fengming, Lyu Yifan, Tan Weihong
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.
Shenzhen Research Institute, Hunan University, Shenzhen, Guangdong 518000, China.
Sci Adv. 2022 Aug 12;8(32):eabq0917. doi: 10.1126/sciadv.abq0917. Epub 2022 Aug 10.
Natural cells (NCs) can automatically and continuously respond to fluctuant external information and distinguish meaningful stimuli from weak noise depending on their powerful genetic and protein networks. We herein report a network topology-directed design of dynamic molecular processing system (DMPS) as a molecular central processing unit that powers an artificial cell (AC) able to process fluctuant information in its immediate environment similar to NCs. By constructing a mixed cell community, ACs and NCs have synchronous response to fluctuant extracellular stimuli under physiological condition and in a blood vessel-mimic circulation system. We also show that fluctuant bioinformation released by NCs can be received and processed by ACs. The molecular design of DMPS-powered AC is expected to allow a profound understanding of biological systems, advance the construction of intelligent molecular systems, and promote more elegant bioengineering applications.
天然细胞（NCs）能够根据其强大的基因和蛋白质网络，自动且持续地响应波动的外部信息，并从微弱噪声中区分出有意义的刺激。我们在此报告了一种动态分子处理系统（DMPS）的网络拓扑导向设计，该系统作为分子中央处理单元，为一种人造细胞（AC）提供动力，使其能够像天然细胞一样处理其周围环境中的波动信息。通过构建混合细胞群落，人造细胞和天然细胞在生理条件下以及在模拟血管的循环系统中对波动的细胞外刺激具有同步响应。我们还表明，天然细胞释放的波动生物信息能够被人造细胞接收和处理。由DMPS驱动的人造细胞的分子设计有望促进对生物系统的深入理解，推动智能分子系统的构建，并促进更出色的生物工程应用。
