Zhang Jing, Cheng Jintang, Yan Liu, Yu Yuetong, Hao Chenyang, Zhao Anyi, Chen Sha, Liu An
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Nanxiaojie, Dongzhimennei, Beijing, 100700, China.
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Nanxiaojie, Dongzhimennei, Beijing, 100700, China.
Phytochemistry. 2022 Nov;203:113355. doi: 10.1016/j.phytochem.2022.113355. Epub 2022 Aug 7.
Ginkgolides are the most important bioactive components of Ginkgo biloba L, of which ginkgolide B has been successfully developed and marketed as a drug. The reported ginkgolides are very rare and exhibit a complex matrix due to the chemodiversity of Ginkgo biloba L. Herein, the global profile of characteristic ion and neutral loss recognition strategy were used for to discover eight undescribed ginkgolides, very rare cyclohexane ginkgolides R-V, ginkgolides D-F, and eight known ginkgolides. These ginkgolides were target isolated and identified using high-resolution mass spectrometry, nuclear magnetic resonance spectroscopy, and X-ray crystallography. The undescribed and known ginkgolides exhibited antiplatelet aggregation activities. In particular, compounds U and D had IC values of 2.20 ± 0.15 and 6.50 ± 0.87 μM, respectively. This study has enriched the known structural diversity of ginkgolides and extended the application of mass spectrometry to the global profiling of natural products present in Ginkgo biloba L. Moreover, it could help chemists rapidly discover unreported compounds from a complex matrix.
银杏内酯是银杏叶中最重要的生物活性成分，其中银杏内酯B已成功开发并作为药物上市。已报道的银杏内酯非常稀少，并且由于银杏叶的化学多样性而呈现出复杂的基质。在此，利用特征离子和中性丢失识别策略的全局概况来发现8种未描述的银杏内酯、非常稀有的环己烷银杏内酯R - V、银杏内酯D - F以及8种已知的银杏内酯。使用高分辨率质谱、核磁共振光谱和X射线晶体学对这些银杏内酯进行了靶向分离和鉴定。未描述的和已知的银杏内酯均表现出抗血小板聚集活性。特别是，化合物U和D的IC值分别为2.20±0.15和6.50±0.87μM。本研究丰富了已知的银杏内酯结构多样性，并将质谱的应用扩展到银杏叶中天然产物的全局概况分析。此外，它可以帮助化学家从复杂的基质中快速发现未报道的化合物。
