Department of Highly Advanced Oral Stomatology, Yokohama Clinic, Kanagawa Dental University, 3-31-6 Tsuruya-cho, Kanagawa, Yokohama, Kanagawa, 221-0835, Japan.
Department of Gastroenterology and Hepatology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan.
Sci Rep. 2022 Aug 10;12(1):13621. doi: 10.1038/s41598-022-17917-2.
The risk factors for non-alcoholic fatty liver disease (NAFLD) progression are not completely known. Porphyromonas gingivalis infection is a risk factor for systemic diseases. We investigated the association of P. gingivalis infection with the risk of non-alcoholic steatohepatitis progression. Here, hematological tests, periodontal examination, and saliva collection were performed for 164 patients with NAFLD. P. gingivalis was identified in saliva using polymerase chain reaction. Hepatic steatosis and stiffness were evaluated using vibration-controlled transient elastography (VCTE) and magnetic resonance imaging. In patients with NAFLD, P. gingivalis positivity (P. gingivalis ratio ≥ 0.01%) in saliva correlated with liver stiffness determined using magnetic resonance elastography (MRE; p < 0.0001). A P. gingivalis ratio of 0.01% corresponds to 100,000 cells/mL and indicates the proportion of P. gingivalis in the total number of bacteria in the oral cavity. Patients with NAFLD and advanced fibrosis on MRE showed significantly elevated endotoxin activity; those who had > 10 periodontal pockets with depths ≥ 4 mm had significantly increased hepatic stiffness on both VCTE and MRE.
非酒精性脂肪性肝病（NAFLD）进展的危险因素尚不完全清楚。牙龈卟啉单胞菌感染是全身性疾病的危险因素。我们研究了牙龈卟啉单胞菌感染与非酒精性脂肪性肝炎进展风险的关系。在这里，对 164 名 NAFLD 患者进行了血液学检查、牙周检查和唾液采集。使用聚合酶链反应在唾液中鉴定出牙龈卟啉单胞菌。使用振动控制瞬态弹性成像（VCTE）和磁共振成像评估肝脂肪变性和硬度。在 NAFLD 患者中，唾液中牙龈卟啉单胞菌阳性（牙龈卟啉单胞菌比值≥0.01%）与磁共振弹性成像（MRE）确定的肝硬度相关（p<0.0001）。牙龈卟啉单胞菌比值为 0.01% 相当于 100,000 个细胞/mL，表明牙龈卟啉单胞菌在口腔内细菌总数中的比例。MRE 显示有晚期纤维化的 NAFLD 患者内毒素活性显著升高；那些有≥4 毫米深度的≥10 个牙周袋的患者在 VCTE 和 MRE 上均有明显升高的肝硬度。
